Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.
Aix Marseille Univ, IRD, APHM, SSA, VITROME, IHU-Méditerranée Infection, Marseille, France.
Biol Direct. 2019 Feb 26;14(1):5. doi: 10.1186/s13062-019-0234-0.
In the recent years, genomic and pan-genomic studies have become increasingly important. Culturomics allows to study human microbiota through the use of different culture conditions, coupled with a method of rapid identification by MALDI-TOF, or 16S rRNA. Bacterial taxonomy is undergoing many changes as a consequence. With the help of pan-genomic analyses, species can be redefined, and new species definitions generated.
Genomics, coupled with culturomics, has led to the discovery of many novel bacterial species or genera, including Akkermansia muciniphila and Microvirga massiliensis. Using the genome to define species has been applied within the genus Klebsiella. A discontinuity or an abrupt break in the core/pan-genome ratio can uncover novel species.
Applying genomic and pan-genomic analyses to the reclassification of other bacterial species or genera will be important in the future of medical microbiology. The pan-genome is one of many new innovative tools in bacterial taxonomy.
This article was reviewed by William Martin, Eric Bapteste and James Mcinerney.
Reviewed by William Martin, Eric Bapteste and James Mcinerney.
近年来，基因组学和泛基因组学研究变得越来越重要。文化组学允许通过使用不同的培养条件，并结合 MALDI-TOF 或 16S rRNA 的快速鉴定方法来研究人类微生物群。因此，细菌分类学正在发生许多变化。借助泛基因组分析，可以重新定义物种，并生成新的物种定义。
基因组学与文化组学的结合导致了许多新型细菌物种或属的发现，包括阿克曼氏菌粘液亚种和马西利亚微菌属。在属内使用基因组定义物种的方法已应用于克雷伯氏菌属。核心/泛基因组比率的不连续或突然中断可以揭示新的物种。
未来医学微生物学中，应用基因组学和泛基因组分析对其他细菌物种或属的重新分类将非常重要。泛基因组是细菌分类学的众多新创新工具之一。
本文由 William Martin、Eric Bapteste 和 James Mcinerney 进行了评审。
由 William Martin、Eric Bapteste 和 James Mcinerney 进行了评审。
