Yoshida T, Suzutani T, Azuma M
Department of Microbiology, Asahikawa Medical College, Japan.
Tohoku J Exp Med. 1988 Nov;156(3):279-90. doi: 10.1620/tjem.156.279.
The appearance of drug resistant strains of herpes simplex virus type 1 (HSV-1) against 9-beta-D-arabinofuranosyladenine (araA), in comparison with 5-iodo-2'-deoxyuridine (IUdR) and 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine, ACG), has been investigated in green monkey kidney cell cultures (Vero) and human embryo lung cell cultures (HEL). Growth curves of HSV-1 in Vero cells and HEL cells in the presence or absence of 20 micrograms/ml of araA showed that not only the viruses produced by these cells in the presence of araA but also the viruses produced in the absence of araA were resistant to the same concentration of araA. However, both viruses were unable to grow in the presence of a combination of 20 micrograms/ml of araA and 3 micrograms/ml of erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA) which is an inhibitor of adenosine deaminase. On the other hand, the viruses produced only in the presence of IUdR or ACG were resistant to each drug. All virus clones obtained from five serial clonings in the presence of araA alone or araA and EHNA in combination were not resistant to araA. These results show that the strain of HSV-1 resistant to araA is an apparently resistant strain, and a truly resistant strain to araA may not be established as easily as the truly resistant strains to IUdR and ACG.
在绿猴肾细胞培养物（Vero）和人胚肺细胞培养物（HEL）中，研究了1型单纯疱疹病毒（HSV-1）对9-β-D-阿拉伯呋喃糖基腺嘌呤（araA）的耐药菌株的出现情况，并与5-碘-2'-脱氧尿苷（IUdR）和9-（2-羟基乙氧基甲基）鸟嘌呤（阿昔洛韦，ACG）进行了比较。在存在或不存在20微克/毫升araA的情况下，HSV-1在Vero细胞和HEL细胞中的生长曲线表明，不仅这些细胞在araA存在下产生的病毒，而且在araA不存在下产生的病毒对相同浓度的araA均具有抗性。然而，当存在20微克/毫升araA和3微克/毫升的红细胞-9-（2-羟基-3-壬基）腺嘌呤（EHNA，一种腺苷脱氨酶抑制剂）的组合时，两种病毒均无法生长。另一方面，仅在IUdR或ACG存在下产生的病毒对每种药物具有抗性。从仅存在araA或araA与EHNA组合的情况下进行的五次连续克隆中获得的所有病毒克隆均对araA不耐药。这些结果表明，对araA耐药的HSV-1菌株是一种明显耐药的菌株，与对IUdR和ACG真正耐药的菌株相比，对araA真正耐药的菌株可能不容易建立。
