Wang Zi-Juan, Chen Xiao-Feng, Zhang Zi-Xiao, Li Yu-Chen, Deng Juan, Tu Jing, Song Zhi-Qiang, Zou Qing-Hua
Department of Microbiology, School of Basic Medical Sciences, Peking University Health Science Centre, Beijing, China.
Peking University Third Hospital, China.
Microb Pathog. 2017 Aug;109:156-161. doi: 10.1016/j.micpath.2017.05.035. Epub 2017 May 25.
The microbiota within humans maintains homeostasis and plays important roles in human health. However, some situations such as the use of antibiotics may disrupt the microbiota balance and result in a series of adverse effects. This study aimed to investigate the effects of a commonly used anti-Helicobacter pylori concomitant therapy on the composition of the gut and throat microbiota and any antibiotic resistance that may develop. In addition to the standard regimen, two different supplementary probiotic regimens that both used Saccharomyces boulardii were included. Microbiological culture-based techniques were used to analyse the microbiota composition and antibiotic resistance. Our results showed marked quantitative and qualitative alterations in both the gut and throat microbiota after treatment with not only the standard concomitant therapy but also with either supplementary probiotic regimen. Nevertheless, most of the changes in the gut microbiota (except for yeast and Bacteroides spp. counts) reverted by Day 71, whereas the alterations in the throat microbiota appeared to persist. Patients treated with the eradication therapy in the absence of probiotic supplementation experienced the most pronounced disturbances in the throat microbiota, whereas changes in the throat microbiota appeared to stabilize in the groups that received probiotic supplementation. We also detected higher antibiotic resistance rates for Enterobacteriaceae, Enterococcus spp. and Bacteroides spp. after treatment with the eradication therapy. Co-administration of probiotics is likely to be more effective than post-antibiotic supplementation, and although some beneficial effects were observed, the probiotic combination did not exert significant effects on the unbalanced commensal gut and throat microbiota composition.
人体内的微生物群维持着体内平衡，并在人类健康中发挥重要作用。然而，某些情况（如使用抗生素）可能会破坏微生物群平衡，并导致一系列不良反应。本研究旨在调查一种常用的抗幽门螺杆菌联合疗法对肠道和咽喉微生物群组成以及可能产生的任何抗生素耐药性的影响。除了标准治疗方案外，还纳入了两种均使用布拉氏酵母菌的不同补充益生菌方案。采用基于微生物培养的技术分析微生物群组成和抗生素耐药性。我们的结果显示，不仅标准联合疗法，而且两种补充益生菌方案治疗后，肠道和咽喉微生物群在数量和质量上均出现了显著变化。尽管如此，肠道微生物群的大多数变化（酵母和拟杆菌属计数除外）在第71天恢复，而咽喉微生物群的变化似乎持续存在。在没有补充益生菌的情况下接受根除治疗的患者，其咽喉微生物群受到的干扰最为明显，而在接受益生菌补充的组中，咽喉微生物群的变化似乎趋于稳定。我们还检测到根除治疗后肠杆菌科、肠球菌属和拟杆菌属的抗生素耐药率较高。益生菌联合使用可能比抗生素后补充更有效，虽然观察到了一些有益效果，但益生菌组合对失衡的共生肠道和咽喉微生物群组成没有显著影响。
