Rejas M T, Rojo J M, Ojeda G, Barasoain I
Department of Immunology, Centro de Investigaciones Biológicas, Madrid, Spain.
Immunopharmacology. 1988 Nov-Dec;16(3):191-7. doi: 10.1016/0162-3109(88)90007-0.
The in vivo effect of sodium diethyldithiocarbamate (DTC) on IL-2 production, mitogen-induced proliferation and natural killer (NK) activity of lymphocytes from normal as well as cyclophosphamide (CY)-treated mice has been investigated. DTC was given in a single subcutaneous injection (25 mg/kg) to normal or cyclophosphamide-treated mice (250 mg/kg i.p. simultaneously to DTC). An enhancement of T lymphocyte proliferation in CY-treated animals and an increase of IL-2 production in both normal and immunosuppressed mice was observed. When DTC (10 mg/kg) was administered daily for two weeks an increase in concanavalin A-induced mitogenesis, IL-2 production and NK activity in CY-treated animals was observed. These immune parameters were reduced 12 days after CY treatment by a factor of 2 to 3 times, while DTC treatment restored these responses to normal levels. LPS-induced mitogenesis was not significantly enhanced. The effect of DTC could be partially mediated by changes in IL-2 activity. According to these results some functional parameters of the immune system of the suppressed host can be partially or completely restored by means of an appropriate immunomodulator treatment. DTC could be of interest in the treatment of diseases where immune functions are impaired or in combined treatments with immunosuppressants.
已研究了二乙基二硫代氨基甲酸钠（DTC）对正常小鼠以及经环磷酰胺（CY）处理的小鼠淋巴细胞白细胞介素-2（IL-2）产生、丝裂原诱导的增殖和自然杀伤（NK）活性的体内作用。将DTC以单次皮下注射（25mg/kg）给予正常或经环磷酰胺处理的小鼠（腹腔注射250mg/kg，与DTC同时给药）。观察到在经CY处理的动物中T淋巴细胞增殖增强，并且在正常和免疫抑制小鼠中IL-2产生增加。当连续两周每天给予DTC（10mg/kg）时，观察到经CY处理的动物中伴刀豆球蛋白A诱导的有丝分裂、IL-2产生和NK活性增加。这些免疫参数在CY处理后12天降低了2至3倍，而DTC处理将这些反应恢复到正常水平。脂多糖诱导的有丝分裂未显著增强。DTC的作用可能部分由IL-2活性的变化介导。根据这些结果，通过适当的免疫调节剂治疗，受抑制宿主免疫系统的一些功能参数可以部分或完全恢复。DTC在治疗免疫功能受损的疾病或与免疫抑制剂联合治疗中可能具有意义。
