Sha'afi R I, Molski T F
Department of Physiology, University of Connecticut Health Center, Farmington 06032.
Membr Biochem. 1987;7(3):143-52. doi: 10.3109/09687688709039991.
The addition of the protein kinase C activator phorbol esters to cell suspension a few minutes prior to stimulation inhibits the agonists-induced biochemical changes and cell responses. This inhibition is prevented by protein kinase C inhibitors. Activation of protein kinase C down regulates the stimulated responses by affecting one or more of the steps in the exitation-response coupling. This includes the receptors, the quanine-nucleotide-binding protein, the activity or distribution of phospholipase C, and other steps.
在刺激前几分钟向细胞悬液中添加蛋白激酶C激活剂佛波酯可抑制激动剂诱导的生化变化和细胞反应。蛋白激酶C抑制剂可阻止这种抑制作用。蛋白激酶C的激活通过影响兴奋-反应偶联中的一个或多个步骤来下调刺激反应。这包括受体、鸟嘌呤核苷酸结合蛋白、磷脂酶C的活性或分布以及其他步骤。
