Mansour Amira Ibrahim, Abd Almonaem Eman Rateb, Behairy Ola Galal, Gouda Tahany Mahmoud
a Departments of Clinical and Chemical Pathology , Benha University , Benha , Egypt.
b Pediatrics , Benha University , Benha , Egypt.
Scand J Clin Lab Invest. 2017 Sep;77(5):373-378. doi: 10.1080/00365513.2017.1328739. Epub 2017 May 30.
This study aimed to evaluate the correlation between serum levels of IL-17 and IL-35 and the presence and severity of childhood asthma. The study was performed on 60 diagnosed asthmatic children, who were further classified into four groups according to the Global Initiative for Asthma Guidelines for Asthma Severity and Control (GINA) 2016, plus 30 age- and sex-matched apparently healthy children. All participants were subjected to full medical history, clinical examination, pulmonary function tests and laboratory evaluation in the form of complete blood count (CBC), serum total IgE, IL-17 and IL-35 by ELISA. Our results revealed that eosinophils count, IgE and IL-17 were significantly higher in the asthmatic group than the control group (p < .001), while IL-35 levels were significantly lower in asthmatics than control (p < .001). A strong negative correlation was found between serum IL-17 and serum IL-35; a positive correlation was found between serum IL-17 and both of serum total IgE and eosinophils counts in atopic asthmatic patients, and serum IL-35 showed significant negative correlations with both. ROC analysis of the data showed that the cut-off value of IL-35 level was <189.5 pg/mL and for IL-17 level, it was >13.1 pg/mL; this value could predict childhood asthma with sensitivity of 81.7% and 83.3%, and specificity of 76.7% and 70%, respectively. A combination of both cytokines yielded an increase in sensitivity to 95%. In conclusion, in the current study, IL-17 is upregulated while IL-35 is downregulated in childhood asthma with a significant negative correlation between both. These results suggest that both may play an important role in the pathogenesis of childhood asthma.
本研究旨在评估血清白细胞介素-17（IL-17）和白细胞介素-35（IL-35）水平与儿童哮喘的存在及严重程度之间的相关性。该研究对60名已确诊的哮喘儿童进行，这些儿童根据2016年全球哮喘防治创议（GINA）哮喘严重程度和控制指南进一步分为四组，另外还有30名年龄和性别匹配的明显健康儿童。所有参与者均接受了全面的病史采集、临床检查、肺功能测试以及实验室评估，包括全血细胞计数（CBC）、血清总免疫球蛋白E（IgE）、通过酶联免疫吸附测定（ELISA）检测的IL-17和IL-35。我们的结果显示，哮喘组的嗜酸性粒细胞计数、IgE和IL-17显著高于对照组（p < 0.001），而哮喘患者的IL-35水平显著低于对照组（p < 0.001）。血清IL-17与血清IL-35之间存在强烈的负相关；在特应性哮喘患者中，血清IL-17与血清总IgE和嗜酸性粒细胞计数均呈正相关，而血清IL-35与两者均呈显著负相关。对数据进行的ROC分析表明，IL-35水平的截断值<189.5 pg/mL，IL-17水平的截断值>13.1 pg/mL；该值可分别以81.7%和83.3%的敏感性以及76.7%和70%的特异性预测儿童哮喘。两种细胞因子联合使用可使敏感性提高至95%。总之，在本研究中，儿童哮喘中IL-17上调而IL-35下调，两者之间存在显著的负相关。这些结果表明，两者可能在儿童哮喘的发病机制中起重要作用。
