Gao Siju, Fan Jingang, Wang Zhaoan
Department of Pediatrics, Linyi People's Hospital, Linyi City, China.
Int Arch Allergy Immunol. 2016;171(3-4):194-202. doi: 10.1159/000452624. Epub 2017 Jan 4.
The aim of this study was to explore whether serum baseline tryptase (sBT) levels might be a useful marker not only for the accurate diagnosis of childhood asthma, but also for the prediction of disease severity.
A total of 114 asthmatic children were enrolled in this study, 36 of whom had mild intermittent asthma, 38 had mild persistent asthma, and 40 had moderate to severe persistent asthma. Additionally, 34 age-matched healthy children were enrolled as controls. The sBT levels of these populations were measured using a fluoroenzymeimmunoassay kit. The diagnostic performance of sBT levels and their correlation with asthma severity were systematically investigated using receiver operating characteristic (ROC) analysis and correlation analysis.
Children with mild and moderate to severe persistent asthma had significantly increased sBT levels as compared to those with mild intermittent asthma and healthy controls. ROC analysis further demonstrated that sBT levels not only appear to be highly sensitive and specific for distinguishing asthmatic children from healthy controls, but also show good accuracy for the differentiation of various asthmatic subgroups. Correlation analysis revealed that in all asthmatic subgroups sBT levels were significantly correlated with a variety of key markers that reflect the disease severity of asthma, including childhood asthma control test scores, serum IgE and interleukin-13 levels, blood eosinophil counts, and pulmonary test parameters.
sBT levels may have a potential use in supporting a diagnosis of asthma in children and as a predictor of disease severity.
本研究的目的是探讨血清基线类胰蛋白酶（sBT）水平是否不仅可作为准确诊断儿童哮喘的有用标志物，还可用于预测疾病严重程度。
本研究共纳入114名哮喘儿童，其中36名患有轻度间歇性哮喘，38名患有轻度持续性哮喘，40名患有中度至重度持续性哮喘。此外，还纳入了34名年龄匹配的健康儿童作为对照。使用荧光酶免疫分析试剂盒测量这些人群的sBT水平。通过受试者工作特征（ROC）分析和相关性分析系统地研究了sBT水平的诊断性能及其与哮喘严重程度的相关性。
与轻度间歇性哮喘儿童和健康对照相比，轻度和中度至重度持续性哮喘儿童的sBT水平显著升高。ROC分析进一步表明，sBT水平不仅似乎对区分哮喘儿童与健康对照具有高度敏感性和特异性，而且对区分各种哮喘亚组也具有良好的准确性。相关性分析显示，在所有哮喘亚组中，sBT水平与反映哮喘疾病严重程度的各种关键标志物显著相关，包括儿童哮喘控制测试评分、血清IgE和白细胞介素-13水平、血液嗜酸性粒细胞计数以及肺功能测试参数。
sBT水平可能在支持儿童哮喘诊断及作为疾病严重程度预测指标方面具有潜在用途。
