Wang Di, Wang Yunlong, Long Wuhua, Niu Mei, Zhao Zhigang, Teng Xuan, Zhu Xiaopin, Zhu Jianping, Hao Yuanyuan, Wang Yongfei, Liu Yi, Jiang Ling, Wang Yihua, Wan Jianmin
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, PR China.
Plant Sci. 2017 Jul;260:90-100. doi: 10.1016/j.plantsci.2017.04.008. Epub 2017 Apr 24.
Tocopherols, a group of Vitamin E compounds, are essential components of the human diet. In contrast to well documented roles in animals, the functions of tocopherols in plants are less understood. In this study, we characterized two allelic rice dwarf mutant lines designated sgd1-1 and sgd1-2 (small grain and dwarf1). Histological observations showed that the dwarf phenotypes were mainly due to cell elongation defects. A map-based cloning strategy and subsequent complementation test showed that SGD1 encodes homogentisate phytyltransferase (HPT), a key enzyme in tocopherol biosynthesis. Mutation of SGD1 resulted in tocopherol deficiency in both sgd1mutants. No oxidant damage was detected in the sgd1 mutants. Further analysis showed that sgd1-2 was hypersensitive to cold stress. Our results indicate that SGD1 is essential for plant development and cold tolerance in rice.
生育酚是一组维生素E化合物，是人类饮食中的必需成分。与在动物体内已得到充分记录的作用相比，生育酚在植物中的功能尚不太清楚。在本研究中，我们对两个等位基因水稻矮化突变体系sgd1-1和sgd1-2（小粒和矮化1）进行了表征。组织学观察表明，矮化表型主要是由于细胞伸长缺陷所致。基于图谱的克隆策略及随后的互补试验表明，SGD1编码尿黑酸植基转移酶（HPT），这是生育酚生物合成中的一种关键酶。SGD1的突变导致两个sgd1突变体中生育酚缺乏。在sgd1突变体中未检测到氧化损伤。进一步分析表明，sgd1-2对冷胁迫高度敏感。我们的结果表明，SGD1对水稻的植株发育和耐寒性至关重要。
