State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, PR China.
Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
Plant Sci. 2017 Aug;261:1-9. doi: 10.1016/j.plantsci.2017.05.005. Epub 2017 May 15.
Numerous studies have demonstrated the function of salinity or jasmonic acid (JA) in plant growth and senescence. This study evaluated how the combination of salinity and methyl jasmonate (MeJA) (SaM) worked as a novel stress and then regulated plant growth in Arabidopsis. Firstly, we found that compared with MeJA or NaCl treatment alone, SaM would significantly intensified plant growth inhibition and senescence in wild-type (WT) seedlings, and these phenotypes could be partially compromised after SaM stress in JA-insensitive mutants. Meanwhile, genes involved in JA signaling and Senescence Associated Gene 13 (SAG13) were dramatically increased by SaM stress than that by MeJA or NaCl alone in WT. Moreover, a group of secondary metabolite - indolic glucosinolates (IGs) showed obvious over-accumulation after SaM treatment than that after each single one in WT, and the seedlings treated with IGs' metabolites performed similar inhibited growth and chlorotic leaves phenotypes compared with those caused by SaM stress. All these indicated the toxicity of IGs and their metabolites would prevent the growth progress of plants. Therefore, we concluded that SaM worked as a novel stress and intensified plant growth inhibition and senescence, which was dependent on JA-dependent and -independent signaling pathways.
许多研究已经证明了盐度或茉莉酸（JA）在植物生长和衰老中的作用。本研究评估了盐度和茉莉酸甲酯（MeJA）（SaM）的组合如何作为一种新型胁迫，然后调节拟南芥中的植物生长。首先，我们发现与 MeJA 或 NaCl 单独处理相比，SaM 会显著加剧野生型（WT）幼苗的生长抑制和衰老，并且这些表型可以在 JA 不敏感突变体中的 SaM 胁迫后部分得到缓解。同时，SaM 胁迫比 MeJA 或 NaCl 单独处理在 WT 中显著增加了参与 JA 信号转导和衰老相关基因 13（SAG13）的基因表达。此外，一组次生代谢物-吲哚类硫葡糖苷（IGs）在 WT 中经 SaM 处理后的积累量明显高于单独处理时的积累量，并且用 IGs 代谢物处理的幼苗表现出与 SaM 胁迫引起的类似的生长抑制和叶片黄化表型。所有这些都表明 IGs 及其代谢物的毒性会阻止植物的生长进程。因此，我们得出结论，SaM 作为一种新型胁迫，通过 JA 依赖和非依赖的信号通路，加剧了植物的生长抑制和衰老。
