Luo Yuqi, Yu Haitao, Ou Wentao, Jia Lin, Huang Yaoxing
Department of General Surgery, Nansha Hospital of Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
Department of General Surgery, Nansha Hospital of Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
Pathol Res Pract. 2017 Jul;213(7):742-748. doi: 10.1016/j.prp.2017.04.008. Epub 2017 Apr 20.
To investigate the characterization of intestinal epithelial stem cells stained by Rhodamine 123 (Rho) and analyze the dynamic changes of intestinal epithelial stem cells during the injured-repaired progress induced by 5-FU.
Mucosal cells were obtained from adult C57BL/6J mice. The Rho stained cells were sorted using FACS. The mouse model of intestinal mucosal injured-repaired was established by injecting 5-FU and sacrificed at different time post-injection, and the middle intestines were used for detecting the percentage of Rho low staining cell fraction by FACS and detecting the expression of the intestinal epithelial stem cells marker-musashi-1 (msi-1) by RT-PCR and immunohistochemistry.
The Rho stained intestinal mucosal cells were divided into three fractions: Rho low staining fraction (12.35%), Rho middle staining fraction (35.5%) and Rho strong staining fraction (50.5%). The cells in Rho low staining fraction expressed rich msi-1 and most of which were in the G0/G1 phase of cell cycle. After treatment of 5-FU, the intestinal mucous were damaged, although the number of msi-1 positive cells has a little decrease, there was no statistical difference among the mice at different time after injection (P>0.05). However, the percentage of msi-1 positive cells increased significantly after injection (P<0.01), and the percentage of msi-1 positive cells decreased gradually during the repaired procedure of the intestinal mucous. There was significant positive correction between the percentage of msi-1 positive cells and the percentage of Rho low staining cell fraction (r=0.867, p<0.01) after 5-FU injection.
The Rho low staining cell fraction from intestinal mucous contained rich intestinal epithelial stem cells, and the intestinal epithelial stem cell which expressed msi-1 played a key role in repairing the damage of intestinal mucous induced by 5-FU.
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