Ferreira Lígia Lavezo, Biasoli Éder Ricardo, Bernabé Daniel Galera, Nunes Cáris Maroni, Miyahara Glauco Issamu
Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, São Paulo State University (UNESP - Univ Estadual Paulista), Araçatuba, São Paulo, 16015-050, Brazil.
Oral Oncology Center and Department of Pathology and Clinical Propaedeutics, Araçatuba School of Dentistry, São Paulo State University (UNESP - Univ Estadual Paulista), Araçatuba, São Paulo, 16015-050, Brazil.
Pathol Res Pract. 2017 Jul;213(7):759-765. doi: 10.1016/j.prp.2017.04.005. Epub 2017 Apr 20.
Oral leukoplakia is considered a potentially malignant lesion for the development of squamous cell carcinoma, and various risk factors may be related to its development and malignant transformation, including the human papillomavirus (HPV). The aim of this case-control study was to detect the presence of HPV in fresh tissue, plasma and saliva samples obtained from patients with and without oral leukoplakia, and verify the correlation of the presence of DNA of HPV between different sources of materials.
In this study, 32 patients with oral leukoplakia and 24 patients selected in a case-control manner were included. DNA extraction from the samples was performed, and afterwards it was amplified by nested polymerase chain reaction (nPCR) for the detection of HPV (nPCR: MY09-MY11/GP05+-GP06+).
The DNA of HPV was found in 68.75% of the fresh tissue samples; in 50% of plasma, and in 62.5% of saliva samples in the group of patients with leukoplakia; in comparison with 45.8%, 54%, and 45.8%, in the fresh tissue, plasma and saliva samples, respectively, in the control group.
Based on the present study, there was no difference in the rate of HPV detection in patients with or without oral leukoplakia. However all sources tested in this study were considered suitable for HPV detection, especially plasma samples, which showed be an important non-invasive source of HPV detection in leukoplakia patients.
口腔白斑被认为是鳞状细胞癌发生的潜在恶性病变，多种风险因素可能与其发生和恶变有关，包括人乳头瘤病毒（HPV）。本病例对照研究的目的是检测从有或无口腔白斑患者获取的新鲜组织、血浆和唾液样本中HPV的存在情况，并验证不同材料来源中HPV DNA存在情况的相关性。
本研究纳入32例口腔白斑患者和24例以病例对照方式选取的患者。对样本进行DNA提取，然后通过巢式聚合酶链反应（nPCR）进行扩增以检测HPV（nPCR：MY09-MY11/GP05+-GP06+）。
白斑患者组中，68.75%的新鲜组织样本、50%的血浆样本和62.5%的唾液样本中发现了HPV DNA；相比之下，对照组的新鲜组织、血浆和唾液样本中HPV DNA的检出率分别为45.8%、54%和45.8%。
基于本研究，有或无口腔白斑患者的HPV检测率无差异。然而，本研究中检测的所有来源都被认为适合HPV检测，尤其是血浆样本，其可能是白斑患者HPV检测的重要非侵入性来源。
