Mathew Asok, Mody R N, Patait Mahendra R, Razooki Ali A, Varghese Nisha T, Saraf Kedar
Department of Surgical Sciences, Ajman University of Science and Technology, Al Fujairah, United Arab Emirates.
Indian J Med Sci. 2011 May;65(5):212-21.
It has been always an area of diffuse clarity when you study malignancy and its pathogenesis. Recently, it has invited lot of interest among the researchers about the possibility of role of viruses in the initiation of carcinogenesis. Recent advances in the field of molecular biology and biotechnology have solved some problems with regard to pathogenesis. Human papilloma virus (HPV) and its role in the initiation of malignancy in the cervix is proven almost beyond doubt.
The present study is aimed at the role of two types of HPV 16 and 18 in the initiation of oral premalignant and squamous cell carcinoma. The study also aims at using polymerase chain reaction (PCR) in finding out the prevalence of these types diagnosed histologically as oral leukoplakia and oral squamous cell carcinoma and prevalence of its association with the habit of tobacco use.
In the present study, 45 patients having histopathologically confirmed oral squamous cell carcinoma in the age range of 32-85 years were selected along with 20 histopathologically confirmed oral leukoplakia in the age range 22-66 years. All the samples were subjected to polymerase chain reaction. The PCR reaction was carried out in PTC 200 thermo-cycler [MJ Research Inc, Watertown, MA, USA].
The site prevalence and co-infection rate of these two types of viruses are being analyzed using very simple non-invasive scrapings obtained from fresh scrapings and found to be really high. It was also observed that 73.3% (33/45) of the oral squamous cell carcinoma patients were positive for oral HPV type 16 while 71.1% (32/45) were positive for HPV type 18 infection and 57.7% (26/45) were found to have both HPV type 16 and HPV type 18 infections.
HPV type 16, 18, and co-infection of both types showed high prevalence in oral squamous cell carcinoma.The prevalence of HPV type 18 was found to be higher than HPV type 16 and co-infection in oral leukoplakia. It was observed that the tongue and palate lesions in the oral squamous cell carcinoma patients showed high prevalence of HPV type 16, type 18, and co-infection compared with other sites.
在研究恶性肿瘤及其发病机制时，这一直是一个模糊不清的领域。最近，病毒在致癌作用起始过程中所起作用的可能性引起了研究人员的极大兴趣。分子生物学和生物技术领域的最新进展解决了一些与发病机制相关的问题。人乳头瘤病毒（HPV）及其在子宫颈恶性肿瘤起始过程中的作用几乎已被证实无疑。
本研究旨在探讨两种HPV类型（16型和18型）在口腔癌前病变和鳞状细胞癌起始过程中的作用。该研究还旨在利用聚合酶链反应（PCR）来查明在组织学上被诊断为口腔白斑和口腔鳞状细胞癌的这些类型的病毒感染率，以及其与吸烟习惯的关联发生率。
在本研究中，选取了45例年龄在32至85岁之间经组织病理学确诊为口腔鳞状细胞癌的患者，以及20例年龄在22至66岁之间经组织病理学确诊为口腔白斑的患者。所有样本均进行聚合酶链反应。PCR反应在PTC 200热循环仪[美国马萨诸塞州沃特敦市MJ研究公司]中进行。
通过从新鲜刮片中获取的非常简单的非侵入性刮片对这两种病毒的部位感染率和共感染率进行分析，发现其感染率确实很高。还观察到，73.3%（33/45）的口腔鳞状细胞癌患者口腔HPV 16型呈阳性反应，71.1%（32/45）的患者HPV 18型感染呈阳性反应，57.7%（26/45）的患者同时感染了HPV 16型和HPV 18型。
HPV 16型、18型以及两种类型的共感染在口腔鳞状细胞癌中显示出高感染率。在口腔白斑中，发现HPV 18型的感染率高于HPV 16型以及共感染率。据观察，与其他部位相比，口腔鳞状细胞癌患者的舌部和腭部病变中HPV 16型、18型以及共感染的发生率较高。
