Yue Zhen, Zhao Zhangwu
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China.
College of Life Science, Linyi University, Linyi, Shandong, China.
Arch Insect Biochem Physiol. 2017 Jun;95(2). doi: 10.1002/arch.21396. Epub 2017 May 30.
The Asian Corn Borer Ostrinia furnacalis is a major agricultural pest. In this study, a full-length neuropeptide Y (npy) gene in O. furnacalis was sequenced and cloned from cDNA library, which contains an ORF of 273 bp by encoding 90 amino acid residues. The mature OfurNPY is composed of 29 amino acids with amidation in C-terminal. The spatiotemporal expression analysis showed that npy highest expression level was in the midgut of the fifth instar larvae (the gluttony period). When the expression of npy was knocked down by feeding or injecting dsNPY, larval food consumption, body size, and body weight were significantly inhibited compared to controls. These results indicate that NPY is an important regulator in the control of feeding of O. furnacalis.
亚洲玉米螟Ostrinia furnacalis是一种主要的农业害虫。在本研究中，从亚洲玉米螟的cDNA文库中对一个全长神经肽Y（npy）基因进行了测序和克隆，该基因包含一个273 bp的开放阅读框，编码90个氨基酸残基。成熟的OfurNPY由29个氨基酸组成，C末端有酰胺化修饰。时空表达分析表明，npy的最高表达水平出现在五龄幼虫（暴食期）的中肠。当通过喂食或注射dsNPY敲低npy的表达时，与对照组相比，幼虫的食物消耗量、体长和体重均受到显著抑制。这些结果表明，NPY是控制亚洲玉米螟取食的重要调节因子。
