Yu Zhuofan, Shi Jian, Jiang Xuemin, Song Yu, Du Juan, Zhao Zhangwu
Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Pest Manag Sci. 2023 Mar;79(3):1193-1203. doi: 10.1002/ps.7289. Epub 2022 Nov 28.
Feeding by pests is one of the most important reasons for reductions in agricultural crop yield. This study aimed to reveal how juvenile hormone (JH) participates in larval feeding regulation of the Asian corn borer Ostrinia furnacalis.
Larvae of O. furnacalis exhibit a daily circadian feeding rhythm, with a peak at ZT18 and a trough at ZT6 under both photoperiod (LD) and constant dark (DD) conditions, which may be eliminated by application of fenoxycarb, a JH active analogue. JH negatively regulates larval feeding as a downstream factor of neuropeptide F (NPF), in which knocking down JH increases larval feeding amount along with body weight and length. The production of JH in the brain-corpora cardiaca-corpora allata (brain-CC-CA) is regulated by brain NPF rather than gut NPF, which was demonstrated in Drosophila larvae through GAL4/UAS genetic analysis. In addition, feeding regulation of JH is closely related to energy homeostasis in the fat body by inhibiting energy storage and promoting degradation. The JH analogue fenoxycarb is an effective pesticide against O. furnacalis, controlling feeding and metabolism.
The brain NPF system regulates JH, with functions in food consumption, feeding rhythms, energy homeostasis and body size. This study provides an important basis for understanding the feeding mechanism and potential pest control of O. furnacalis. © 2022 Society of Chemical Industry.
害虫取食是导致农作物产量下降的最重要原因之一。本研究旨在揭示保幼激素（JH）如何参与亚洲玉米螟Ostrinia furnacalis幼虫的取食调控。
在光周期（LD）和持续黑暗（DD）条件下，亚洲玉米螟幼虫均表现出每日的昼夜取食节律，在ZT18时达到峰值，在ZT6时出现低谷，而施用JH活性类似物苯氧威可消除这种节律。JH作为神经肽F（NPF）的下游因子对幼虫取食起负调控作用，敲低JH会增加幼虫的取食量以及体重和体长。通过GAL4/UAS基因分析在果蝇幼虫中证实，脑-心侧体-咽侧体（脑-CC-CA）中JH的产生受脑NPF而非肠道NPF的调控。此外，JH的取食调控通过抑制能量储存和促进降解与脂肪体中的能量稳态密切相关。JH类似物苯氧威是一种防治亚洲玉米螟的有效杀虫剂，可控制其取食和代谢。
脑NPF系统调控JH，在食物消耗、取食节律、能量稳态和体型方面发挥作用。本研究为理解亚洲玉米螟的取食机制和潜在害虫防治提供了重要依据。© 2022化学工业协会。
