Department of Otolaryngology-Head and Neck Surgery, Seoul National University Boramae Medical Center , 5 Gil 20, Boramae-ro, Dongjak-Gu, Seoul 156-707, Republic of Korea.
Department of Chemistry, College of Natural Sciences, Seoul National University , 1 Gwanak-ro, Gwanak-gu, Seoul 151-747, Republic of Korea.
ACS Appl Mater Interfaces. 2017 Jun 7;9(22):19161-19175. doi: 10.1021/acsami.7b02696. Epub 2017 May 30.
Expanded polytetrafluoroethylene (ePTFE), also known as Gore-Tex, is widely used as an implantable biomaterial in biomedical applications because of its favorable mechanical properties and biochemical inertness. However, infection and inflammation are two major complications with ePTFE implantations, because pathogenic bacteria can inhabit the microsized pores, without clearance by host immune cells, and the limited biocompatibility can induce foreign body reactions. To minimize these complications, we covalently grafted a biomembrane-mimic polymer, poly(2-methacryloyloxylethyl phosphorylcholine) (PMPC), by partial defluorination followed by UV-induced polymerization with cross-linkers on the ePTFE surface. PMPC grafting greatly reduced serum protein adsorption as well as fibroblast adhesion on the ePTFE surface. Moreover, the PMPC-grafted ePTFE surface exhibited a dramatic inhibition of the adhesion and growth of Staphylococcus aureus, a typical pathogenic bacterium in ePTFE implants, in the porous network. On the basis of an analysis of immune cells and inflammation-related factors, i.e., transforming growth factor-β (TGF-β) and myeloperoxidase (MPO), we confirmed that inflammation was efficiently alleviated in tissues around PMPC-grafted ePTFE plates implanted in the backs of rats. Covalent PMPC may be an effective strategy for promoting anti-inflammatory and antibacterial functions in ePTFE implants and to reduce side effects in biomedical applications of ePTFE.
膨体聚四氟乙烯（ePTFE），又称 Gore-Tex，由于其具有优良的机械性能和生化惰性，被广泛用作生物医学应用中的可植入生物材料。然而，感染和炎症是 ePTFE 植入物的两个主要并发症，因为病原体可以栖息在微孔中，而不会被宿主免疫细胞清除，并且有限的生物相容性会引起异物反应。为了最大限度地减少这些并发症，我们通过部分脱氟，然后用交联剂通过紫外线诱导聚合，在 ePTFE 表面共价接枝了一种仿生聚合物聚（2-甲基丙烯酰氧乙基磷酸胆碱）（PMPC）。PMPC 接枝极大地减少了 ePTFE 表面的血清蛋白吸附和成纤维细胞黏附。此外，PMPC 接枝的 ePTFE 表面在多孔网络中对金黄色葡萄球菌（ePTFE 植入物中典型的病原体）的黏附和生长表现出明显的抑制作用。基于对免疫细胞和炎症相关因子（即转化生长因子-β（TGF-β）和髓过氧化物酶（MPO））的分析，我们证实 PMPC 接枝的 ePTFE 板植入大鼠背部组织后，炎症得到了有效缓解。共价接枝的 PMPC 可能是促进 ePTFE 植入物抗炎和抗菌功能的有效策略，并减少 ePTFE 在生物医学应用中的副作用。
