Taouki Ioanna, Tasiudi Eve, Lalioti Maria-Eleni, Kyrousi Christina, Skavatsou Eleni, Kaplani Konstantina, Lygerou Zoi, Kouvelas Elias D, Mitsacos Adamantia, Giompres Panagiotis, Taraviras Stavros
1 Department of Physiology, School of Medicine, University of Patras , Patras, Greece .
2 Department of Physiology, School of Biology, University of Patras , Patras, Greece .
Stem Cells Dev. 2017 Aug 15;26(16):1214-1222. doi: 10.1089/scd.2016.0335. Epub 2017 Jun 30.
Neural stem cells have been considered as a source of stem cells that can be used for cell replacement therapies in neurodegenerative diseases, as they can be isolated and expanded in vitro and can be used for autologous grafting. However, due to low percentages of survival and varying patterns of differentiation, strategies that will enhance the efficacy of transplantation are under scrutiny. In this article, we have examined whether alterations in Geminin's expression, a protein that coordinates the balance between self-renewal and differentiation, can improve the properties of stem cells transplanted in 6-OHDA hemiparkinsonian mouse model. Our results indicate that, in the absence of Geminin, grafted cells differentiating into dopaminergic neurons were decreased, while an increased number of oligodendrocytes were detected. The number of proliferating multipotent cells was not modified by the absence of Geminin. These findings encourage research related to the impact of Geminin on transplantations for neurodegenerative disorders, as an important molecule in influencing differentiation decisions of the cells composing the graft.
神经干细胞被认为是一种可用于神经退行性疾病细胞替代疗法的干细胞来源，因为它们能够在体外分离和扩增，并可用于自体移植。然而，由于存活率低以及分化模式各异，提高移植疗效的策略正在研究之中。在本文中，我们研究了Geminin（一种协调自我更新和分化之间平衡的蛋白质）表达的改变是否能改善在6-OHDA半帕金森病小鼠模型中移植的干细胞的特性。我们的结果表明，在没有Geminin的情况下，分化为多巴胺能神经元的移植细胞减少，而检测到少突胶质细胞数量增加。Geminin的缺失并未改变增殖多能细胞的数量。这些发现鼓励开展有关Geminin对神经退行性疾病移植影响的研究，因为它是影响移植细胞分化决定的一个重要分子。
