Liu Han, Sun Bo, Wang Shengnan, Liu Congjin, Lu Yun, Li Ding, Liu Xingdang
Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, China.
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
Cell Physiol Biochem. 2017;42(1):373-382. doi: 10.1159/000477481. Epub 2017 May 25.
BACKGROUND/AIMS: Circulating tumor cells (CTCs) are valuable in both basic research and clinical application for cancer management. In the current study, we evaluated the diagnostic value of CTCs in pancreatic ductal adenocarcinoma (PDAC).
In total, 143 blood samples from 95 consecutively diagnosed PDAC patients and 48 healthy donors were collected. Combined data from immunostaining of CD45, DAPI and fluorescence in situ hybridization (FISH) with chromosome 8 centromere (CEP8) probe were used to identify CTCs. Cells with features of CD45-/DAPI+/CEP8>2 were detected as CTCs.
CTCs were classified as triploid, tetraploid and multiploid based on chromosome 8 copy number. CTC subtype composition was significantly different among groups. Both subtype number and total CTC number were significantly increased in PDAC patients, compared to healthy controls. Total CTC number had 75.8% sensitivity and 68.7% specificity at a cutoff value of 2 cells/3.2 mL. This study is the first to report that CTC subtype number is also useful in cancer diagnosis. Sensitivity was 53.7% and specificity was 85.4% at a cutoff point of 2 CTC subtypes. The diagnostic value of both total CTC number and CTC subtype number was a little poorer than CA199.
Both CTC subtype and total CTC number may serve as potential biomarkers for PDAC.
背景/目的：循环肿瘤细胞（CTC）在癌症研究的基础研究和临床应用中均具有重要价值。在本研究中，我们评估了CTC在胰腺导管腺癌（PDAC）中的诊断价值。
共收集了95例连续确诊的PDAC患者和48例健康供体的143份血样。结合CD45免疫染色、DAPI和8号染色体着丝粒（CEP8）探针荧光原位杂交（FISH）的数据来鉴定CTC。具有CD45-/DAPI+/CEP8>2特征的细胞被检测为CTC。
根据8号染色体拷贝数，CTC被分为三倍体、四倍体和多倍体。各组间CTC亚型组成存在显著差异。与健康对照相比，PDAC患者的亚型数量和总CTC数量均显著增加。在截断值为2个细胞/3.2 mL时，总CTC数量的敏感性为75.8%，特异性为68.7%。本研究首次报道CTC亚型数量在癌症诊断中也具有作用。在截断点为2个CTC亚型时，敏感性为53.7%，特异性为85.4%。总CTC数量和CTC亚型数量的诊断价值均略低于CA199。
CTC亚型和总CTC数量均可作为PDAC的潜在生物标志物。
