Cañibano-Hernández Alberto, Saenz Del Burgo Laura, Espona-Noguera Albert, Orive Gorka, Hernández Rosa M, Ciriza Jesús, Pedraz Jose Luis
NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country UPV/EHU , Vitoria-Gasteiz 01006, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN , Vitoria-Gasteiz 01006, Spain.
Mol Pharm. 2017 Jul 3;14(7):2390-2399. doi: 10.1021/acs.molpharmaceut.7b00295. Epub 2017 Jun 6.
The potential clinical application of alginate cell microencapsulation has advanced enormously during the past decade. However, the 3D environment created by alginate beads does not mimic the natural extracellular matrix surrounding cells in vivo, responsible of cell survival and functionality. As one of the most frequent macromolecules present in the extracellular matrix is hyaluronic acid, we have formed hybrid beads with alginate and hyaluronic acid recreating a closer in vivo cell environment. Our results show that 1% alginate-0.25% hyaluronic acid microcapsules retain 1.5% alginate physicochemical properties. Moreover, mesenchymal stem cells encapsulated in these hybrid beads show enhanced viability therapeutic protein release and mesenchymal stem cells' potential to differentiate into chondrogenic lineage. Although future studies with additional proteins need to be done in order to approach even more the extracellular matrix features, we have shown that hyaluronic acid protects alginate encapsulated mesenchymal stem cells by providing a niche-like environment and remaining them competent as a sustainable drug delivery system.
在过去十年中，藻酸盐细胞微囊化的潜在临床应用取得了巨大进展。然而，藻酸盐珠粒所营造的三维环境并不能模拟体内细胞周围负责细胞存活和功能的天然细胞外基质。由于细胞外基质中最常见的大分子之一是透明质酸，我们用藻酸盐和透明质酸形成了混合珠粒，从而更接近地重现体内细胞环境。我们的结果表明，1%藻酸盐-0.25%透明质酸微囊保留了1.5%藻酸盐的物理化学性质。此外，封装在这些混合珠粒中的间充质干细胞显示出增强的活力、治疗性蛋白质释放以及间充质干细胞向软骨生成谱系分化的潜力。尽管为了更接近细胞外基质特征还需要对其他蛋白质进行进一步研究，但我们已经表明，透明质酸通过提供类似生态位的环境并使它们作为可持续药物递送系统保持活性，从而保护了藻酸盐封装的间充质干细胞。
