Bai Bin, Wang Le, Lee May, Zhang Yingjun, Alfiko Yuzer, Ye Bao Qing, Wan Zi Yi, Lim Chin Huat, Suwanto Antonius, Chua Nam-Hai, Yue Gen Hua
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.
R & D Department, Wilmar International Plantation, Palembang, Indonesia.
BMC Plant Biol. 2017 May 30;17(1):93. doi: 10.1186/s12870-017-1045-z.
Oil palm (Elaeis guineensis, Jacq.) is the most important source of edible oil. The improvement of oil yield is currently slow in conventional breeding programs due to long generation intervals. Marker-assisted selection (MAS) has the potential to accelerate genetic improvement. To identify DNA markers associated with oil content traits for MAS, we performed quantitative trait loci (QTL) mapping using genotyping by sequencing (GBS) in a breeding population derived from a cross between Deli Dura and Ghana Pisifera, containing 153 F trees.
We constructed a high-density linkage map containing 1357 SNPs and 123 microsatellites. The 16 linkage groups (LGs) spanned 1527 cM, with an average marker space of 1.03 cM. One significant and three suggestive QTL for oil to bunch (O/B) and oil to dry mesocarp (O/DM) were mapped on LG1, LG8, and LG10 in a F breeding population, respectively. These QTL explained 7.6-13.3% of phenotypic variance. DNA markers associated with oil content in these QTL were identified. Trees with beneficial genotypes at two QTL for O/B showed an average O/B of 30.97%, significantly (P < 0.01) higher than that of trees without any beneficial QTL genotypes (average O/B of 28.24%). QTL combinations showed that the higher the number of QTL with beneficial genotypes, the higher the resulting average O/B in the breeding population.
A linkage map with 1480 DNA markers was constructed and used to identify QTL for oil content traits. Pyramiding the identified QTL with beneficial genotypes associated with oil content traits using DNA markers has the potential to accelerate genetic improvement for oil yield in the breeding population of oil palm.
油棕（Elaeis guineensis, Jacq.）是最重要的食用油来源。由于世代间隔长，目前传统育种计划中提高油产量的速度较慢。标记辅助选择（MAS）有加速遗传改良的潜力。为了鉴定与油含量性状相关的DNA标记用于MAS，我们在一个由德利杜拉（Deli Dura）和加纳比西弗拉（Ghana Pisifera）杂交产生的包含153棵F代树的育种群体中，使用简化基因组测序（GBS）进行数量性状位点（QTL）定位。
我们构建了一个包含1357个单核苷酸多态性（SNP）和123个微卫星的高密度连锁图谱。16个连锁群（LG）跨度为1527厘摩（cM），平均标记间距为1.03 cM。在一个F代育种群体中，分别在LG1、LG8和LG10上定位到一个与果穗含油率（O/B）和干中果皮含油率（O/DM）相关的显著QTL和三个暗示性QTL。这些QTL解释了7.6 - 13.3%的表型变异。鉴定出了与这些QTL中油含量相关的DNA标记。在两个与O/B相关的QTL上具有有利基因型的树，其平均O/B为30.97%，显著高于（P < 0.01）没有任何有利QTL基因型的树（平均O/B为28.24%）。QTL组合表明，有利基因型的QTL数量越多，育种群体中最终的平均O/B越高。
构建了一个包含1480个DNA标记的连锁图谱，并用于鉴定油含量性状的QTL。利用DNA标记将鉴定出的QTL与油含量性状相关的有利基因型聚合，有潜力加速油棕育种群体中油产量的遗传改良。
