Wang Le, Wan Zi Yi, Bai Bin, Huang Shu Qing, Chua Elaine, Lee May, Pang Hong Yan, Wen Yan Fei, Liu Peng, Liu Feng, Sun Fei, Lin Grace, Ye Bao Qing, Yue Gen Hua
Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604.
Department of Biological Sciences, National University of Singapore, 14 Science Drive, Singapore 117543.
Sci Rep. 2015 Nov 10;5:16358. doi: 10.1038/srep16358.
A high-density genetic map is essential for comparative genomic studies and fine mapping of QTL, and can also facilitate genome sequence assembly. Here, a high density genetic map of Asian seabass was constructed with 3321 SNPs generated by sequencing 144 individuals in a F2 family. The length of the map was 1577.67 cM with an average marker interval of 0.52 cM. A high level of genomic synteny among Asian seabass, European seabass, Nile tilapia and stickleback was detected. Using this map, one genome-wide significant and five suggestive QTL for growth traits were detected in six linkage groups (i.e. LG4, LG5, LG11, LG13, LG14 and LG15). These QTL explained 10.5-16.0% of phenotypic variance. A candidate gene, ACOX1 within the significant QTL on LG5 was identified. The gene was differentially expressed between fast- and slow-growing Asian seabass. The high-density SNP-based map provides an important tool for fine mapping QTL in molecular breeding and comparative genome analysis.
高密度遗传图谱对于比较基因组研究和数量性状位点（QTL）的精细定位至关重要，还能促进基因组序列组装。在此，利用在一个F2家系中对144个个体进行测序产生的3321个单核苷酸多态性（SNP）构建了尖吻鲈的高密度遗传图谱。该图谱长度为1577.67厘摩（cM），平均标记间隔为0.52厘摩。在尖吻鲈、欧洲鲈鱼、尼罗罗非鱼和棘鱼之间检测到了高水平的基因组共线性。利用该图谱，在六个连锁群（即LG4、LG5、LG11、LG13、LG14和LG15）中检测到一个全基因组显著的和五个暗示性的生长性状QTL。这些QTL解释了10.5 - 16.0%的表型变异。在LG5上显著QTL内鉴定出一个候选基因ACOX1。该基因在快速生长和慢速生长的尖吻鲈之间存在差异表达。基于高密度SNP的图谱为分子育种中QTL的精细定位和比较基因组分析提供了一个重要工具。
Zotero 插件
