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在暴露于交流电的红细胞中观察到的变化。

Changes observed in erythrocyte cells exposed to an alternating current.

作者信息

Jeican Ionut Isaia, Matei Horea, Istrate Alexandru, Mironescu Eugen, Bâlici Ştefana

机构信息

Department of Cell and Molecular Biology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.

出版信息

Clujul Med. 2017;90(2):154-160. doi: 10.15386/cjmed-696. Epub 2017 Apr 25.

DOI:10.15386/cjmed-696
PMID:28559698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5433566/
Abstract

BACKGROUND AND AIMS

Appliance of electric pulses induces red blood cells (RBCs) membrane poration, membrane aminophospholipid perturbation and alteration of the normal flip-flop process, resulting in various shape changes of the RBCs. We studied morphological and water permeability changes of RBCs bombarded with electrons in an alternating current circuit.

METHODS

We used three venous blood samples of 100 mL and an alternating current device. The harvested blood was divided into four experimental sets to be used for various exposure times: 0 hours (control RBCs), 0.5h, 3h and 6h (electric-stimulated RBCs). Following the electric current each of the four sets were further divided into three samples: one for the assessment of the echinocytes/RBCs ratio, another for the electron microscopy study of ultrastructural changes induced by the alternating electrical current and a larger third one for determining water permeability of RCBs by H-NMR spectroscopy and morphological measurements.

RESULTS

There is a small but statistically significant effect of the RBC exposure to alternating electric current on cell diameters. Exposure to electric current is positively and strongly correlated with the percentage of echinocytes in a duration-dependent manner. There is a strong and statistically significant correlation between electric current exposure and permeability to water as measured by H-NMR spectroscopy.

CONCLUSION

Following interactions between electric current and RBC membrane, certain modifications were observed in the erythrocyte structure. We attribute the increased cell size to a higher permeability to water and a decreased tonicity. This leads to the transformation of the RBCs into echinocytes.

摘要

背景与目的

施加电脉冲会诱导红细胞（RBC）膜形成孔隙、膜氨基磷脂紊乱以及正常翻转过程改变，从而导致红细胞出现各种形状变化。我们研究了在交流电路中用电子轰击红细胞后其形态和水渗透性的变化。

方法

我们使用了三份100毫升的静脉血样本和一台交流设备。采集的血液被分成四组实验样本，用于不同的暴露时间：0小时（对照红细胞）、0.5小时、3小时和6小时（电刺激红细胞）。施加电流后，这四组样本每组又进一步分成三个样本：一个用于评估棘形红细胞/红细胞比率，另一个用于对交流电诱导的超微结构变化进行电子显微镜研究，第三个较大的样本用于通过氢核磁共振光谱法测定红细胞的水渗透性和进行形态测量。

结果

红细胞暴露于交流电对细胞直径有微小但具有统计学意义的影响。暴露于电流与棘形红细胞百分比呈正相关且具有强烈的时间依赖性。通过氢核磁共振光谱法测量，电流暴露与水渗透性之间存在强烈且具有统计学意义的相关性。

结论

电流与红细胞膜相互作用后，在红细胞结构中观察到了某些改变。我们将细胞大小增加归因于更高的水渗透性和更低的张力。这导致红细胞转变为棘形红细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/91235dd7d7b7/cm-90-154f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/f9fff3d2c3b9/cm-90-154f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/b2b7cdf49ab7/cm-90-154f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/4215fe9b7a74/cm-90-154f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/c6660aabe891/cm-90-154f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/eb364dfd93ec/cm-90-154f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/e72c41bac576/cm-90-154f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/91235dd7d7b7/cm-90-154f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/f9fff3d2c3b9/cm-90-154f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/491897ffac84/cm-90-154f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/b2b7cdf49ab7/cm-90-154f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/4215fe9b7a74/cm-90-154f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/c6660aabe891/cm-90-154f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/eb364dfd93ec/cm-90-154f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/e72c41bac576/cm-90-154f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1523/5433566/91235dd7d7b7/cm-90-154f8.jpg

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J Biomed Opt. 2012 Oct;17(10):106013. doi: 10.1117/1.JBO.17.10.106013.
3
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Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6731-6. doi: 10.1073/pnas.0909533107. Epub 2010 Mar 29.
4
[Transformations of erythrocytes shape and its regulation].[红细胞形状的转变及其调节]
Postepy Biochem. 2009;55(4):425-33.
5
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Bioelectrochemistry. 2010 Feb;77(2):158-61. doi: 10.1016/j.bioelechem.2009.07.006. Epub 2009 Jul 21.
6
Effects of electric fields on proton transport through water chains.电场对质子通过水链传输的影响。
J Chem Phys. 2006 May 28;124(20):204510. doi: 10.1063/1.2198820.
7
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