Sharp J M, Littlejohns I R, St George T D
New South Wales Department of Agriculture, Glenfield.
Aust J Biol Sci. 1988;41(4):553-62. doi: 10.1071/bi9880553.
Using antigens prepared from cell cultures infected by bluetongue (BLU) virus type 20 (BLU-20), and sera from cattle which had recovered from experimental infection by that virus, two distinct precipitin reactions were demonstrated by immunodiffusion. Two distinct gel diffusion precipitin tests were developed based on these reactions. The antigen of one was common to BLU-20 and two other Australian BLU isolates, CSIRO 154 (BLU-21) and CSIRO 156 (BLU-1). It was therefore concluded to be a group-specific test. The antigen of the second appeared to be unique to BLU-20. The test based on this antigen correlated well with the virus neutralization test for BLU-20 and it was therefore concluded to be type-specific. Similar methods applied to a virus of the Palyam (PAL) group demonstrated two precipitin reactions of similar broad (group) and narrow (type) specificity.
利用从感染20型蓝舌病（BLU）病毒（BLU - 20）的细胞培养物中制备的抗原，以及从经该病毒实验性感染后康复的牛血清，通过免疫扩散法显示出两种不同的沉淀反应。基于这些反应开发了两种不同的凝胶扩散沉淀试验。其中一种试验的抗原是BLU - 20以及另外两种澳大利亚蓝舌病分离株CSIRO 154（BLU - 21）和CSIRO 156（BLU - 1）所共有的。因此得出结论，这是一种群特异性试验。第二种试验的抗原似乎是BLU - 20所特有的。基于该抗原的试验与BLU - 20的病毒中和试验相关性良好，因此得出结论，这是一种型特异性试验。将类似方法应用于帕利亚姆（PAL）组病毒，显示出两种具有相似宽泛（群）特异性和狭窄（型）特异性的沉淀反应。
