Grimes J E, McConnell S, Livingston C W, Unger C L
Vet Microbiol. 1983 Aug;8(4):363-72. doi: 10.1016/0378-1135(83)90049-4.
Three methods of extracting bluetongue virus (BTV)-infected newborn mouse brains to prepare immunodiffusion (ID) test antigen were used. The most readily readable and reproducible results were obtained with fluorocarbon-extracted brains homogenized in 8.5% sucrose. Mouse brain- and reference cell culture-derived antigens gave a line of identity with anti-BTV serum. Extracts of noninfected brains were nonreactive. ID tests on field-collected bovine sera, comparing the two types of antigen, resulted in only 73% agreement due to a greater sensitivity of cell culture-derived antigen. A 70.5% agreement resulted when comparing mouse brain-derived antigen in ID tests with complement fixation tests, the latter being least sensitive. ID test results with sera from experimental sheep gave 95.9% agreement between cell culture- and mouse brain-derived antigens. Between ID, which detects antibody to the BTV common or group antigen, and virus neutralization, which detects type-specific antibody, the agreement was 71.4% with postchallenge sera. Data from pre- and postinjection sera, however, indicate the possible activity in Texas of viruses other than International BTV Types 10, 11, 13, and 17.
采用了三种从感染蓝舌病病毒（BTV）的新生小鼠大脑中提取免疫扩散（ID）试验抗原的方法。用氟碳提取的大脑在8.5%蔗糖中匀浆得到的结果最易于读取且可重复。小鼠脑源性抗原和参考细胞培养源性抗原与抗BTV血清形成一条同一线。未感染大脑的提取物无反应。对现场采集的牛血清进行ID试验，比较两种抗原，由于细胞培养源性抗原的敏感性更高，一致性仅为73%。在ID试验中，将小鼠脑源性抗原与补体结合试验进行比较时，一致性为70.5%，后者敏感性最低。用实验绵羊血清进行ID试验的结果显示，细胞培养源性抗原和小鼠脑源性抗原之间的一致性为95.9%。在检测BTV共同或群抗原抗体的ID试验和检测型特异性抗体的病毒中和试验之间，攻毒后血清的一致性为71.4%。然而，注射前和注射后血清的数据表明，在得克萨斯州可能存在国际BTV 10型、11型、13型和17型以外的病毒活性。
