Genest D, Mazeau K, Ptak M
Centre de Biophysique Moléculaire, C.N.R.S., Orléans, France.
J Biomol Struct Dyn. 1987 Aug;5(1):67-78. doi: 10.1080/07391102.1987.10506375.
The Z conformation of the auto complementary hexanucleoside pentaphosphate d(br5C-G)3 in 1 M NaClO4 solution has been investigated by using 2D NMR techniques. NOESY experiments performed at different temperatures show that the oligonucleotide exhibits end-to-end associations at room temperature. The conformation of the hexanucleotide molecules is very similar to that found in the crystal which was described by Chevrier et al. (J. Mol. Biol., 1986, 188, 707-719) as a Z-I form. When the temperature is increased the aggregates are dissociated and a conformational change is observed which is interpreted as a Z-I in equilibrium Z-II transition.
利用二维核磁共振技术研究了在1 M高氯酸钠溶液中自互补六核苷酸五磷酸d(br5C-G)3的Z构象。在不同温度下进行的核欧沃豪斯效应光谱实验表明，该寡核苷酸在室温下呈现端对端缔合。六核苷酸分子的构象与Chevrier等人（《分子生物学杂志》，1986年，第188卷，707 - 719页）描述为Z-I形式的晶体中的构象非常相似。当温度升高时，聚集体解离，观察到构象变化，这被解释为Z-I向Z-II的平衡转变。
