消除DNA端对端堆积相互作用
Closing the lid on DNA end-to-end stacking interactions.
作者信息
Li Li, Pabit Suzette A, Lamb Jessica S, Park Hye Yoon, Pollack Lois
机构信息
School of Applied and Engineering Physics, Cornell University, Ithaca, New York 14853, USA.
出版信息
Appl Phys Lett. 2008 Jun 2;92(22):223901-2239013. doi: 10.1063/1.2937402.
Abstract
Recent experiments suggest that short DNA strands associate by end-to-end stacking. Here, we report interactions between DNAs with modified ends. DNA duplexes, 20 bp long, were capped with short T(4) loops at 2, 1 or 0 ends, and were placed in solutions containing 20 mM Mg(2+). Association was observed only in constructs with one or more uncapped ends. DNA-DNA interactions were characterized by measuring variations in small angle x-ray scattering (SAXS) curves at the lowest scattering angles. Second virial coefficients were computed from the SAXS data. Our results confirm that end-to-end stacking plays an important role in short strand DNA-DNA interactions.
摘要
近期实验表明，短DNA链通过端对端堆积相互结合。在此，我们报告了具有修饰末端的DNA之间的相互作用。20个碱基对长的DNA双链体在2个、1个或0个末端被短T(4)环封闭，并置于含有20 mM Mg(2+)的溶液中。仅在具有一个或多个未封闭末端的构建体中观察到结合。通过测量最低散射角处小角X射线散射（SAXS）曲线的变化来表征DNA-DNA相互作用。从SAXS数据计算第二维里系数。我们的结果证实，端对端堆积在短链DNA-DNA相互作用中起重要作用。
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