Ernfors P, Hallböök F, Ebendal T, Shooter E M, Radeke M J, Misko T P, Persson H
Department of Medical Chemistry, Karolinska Institute, Stockholm, Sweden.
Neuron. 1988 Dec;1(10):983-96. doi: 10.1016/0896-6273(88)90155-9.
Hybridization probes from the transmembrane region of the chick NGF receptor (NGF-R) that show high homology with the rat NGF-R were used to demonstrate an abundant 4.5 kb NGF-R mRNA in the chick embryo at E3.5. The level remained high until E12 but decreased to adult levels by E18. The highest levels at E8 were in spinal cord, bursa of Fabricius, gizzard, femoralis muscle, and skin. In situ hybridization to E7 embryos showed high expression of the NGF-R gene in spinal cord, particularly the lateral motor column, and in dorsal root, sympathetic, and nodose ganglia. NGF-R mRNA expression was observed throughout brain development and in all regions of the adult brain, with high levels in cerebellum and septum. Lymphoid tissues of chick and rat also expressed the receptor. The complex and widespread expression of NGF-R mRNA in areas not known to be NGF targets suggests broader functions for NGF.
来自鸡神经生长因子受体（NGF-R）跨膜区域的杂交探针与大鼠NGF-R具有高度同源性，被用于证明在胚胎第3.5天（E3.5）的鸡胚中存在丰富的4.5 kb NGF-R mRNA。该水平在E12之前一直保持较高，但到E18时降至成年水平。E8时最高水平出现在脊髓、法氏囊、砂囊、股肌和皮肤中。对E7胚胎进行原位杂交显示，NGF-R基因在脊髓中高表达，尤其是外侧运动柱，以及背根神经节、交感神经节和结状神经节。在整个脑发育过程以及成体脑的所有区域均观察到NGF-R mRNA表达，在小脑和隔区水平较高。鸡和大鼠的淋巴组织也表达该受体。NGF-R mRNA在未知为NGF靶标的区域中复杂且广泛的表达表明NGF具有更广泛的功能。
