Heuer J G, Fatemie-Nainie S, Wheeler E F, Bothwell M
Department of Physiology & Biophysics, School of Medicine, University of Washington, Seattle 98195.
Dev Biol. 1990 Feb;137(2):287-304. doi: 10.1016/0012-1606(90)90255-h.
The nucleotide and deduced amino acid sequence of a cDNA clone of the chicken NGF receptor (NGFR) is reported and is compared with sequences of mammalian NGF receptors. A model is presented in which monodentate or bidentate binding of NGF dimers to repeated cysteine-rich sequence elements of the receptor yields low- or high-affinity NGF binding, respectively. In situ hybridization is used to characterize expression of NGFR in developing chick from 40 hr to 10 days of embryogenesis. NGFR mRNA expression is detected in premigratory neural crest cells, in epibranchial placode cells, and in all sensory, sympathetic and parasympathetic derivatives of these structures. In the embryonic CNS, NGFR mRNA is detected in the mantle zone but not the periventricular germinal zone throughout most of the neural tube. By Embryonic Day 8, NGFR mRNA is detected in a substantial fraction of cells in every brain region, with highest levels present in developing motor neurons. NGFR mRNA also is transiently expressed in many mesenchymal cell populations including cells in branchial arch, sclerotome, muscle anlagen, and feather follicles. The functional significance of wide-spread embryonic expression of the NGF receptor is discussed.
报道了鸡神经生长因子受体（NGFR）cDNA克隆的核苷酸和推导的氨基酸序列，并与哺乳动物NGFR的序列进行了比较。提出了一个模型，其中神经生长因子二聚体与受体重复的富含半胱氨酸的序列元件的单齿或双齿结合分别产生低亲和力或高亲和力的神经生长因子结合。原位杂交用于表征从胚胎发生40小时到10天的发育中的鸡中NGFR的表达。在迁移前的神经嵴细胞、鳃上神经节细胞以及这些结构的所有感觉、交感和副交感衍生物中检测到NGFR mRNA表达。在胚胎中枢神经系统中，在神经管的大部分区域，在套层区而非脑室周围生发区检测到NGFR mRNA。到胚胎第8天，在每个脑区的大部分细胞中检测到NGFR mRNA，在发育中的运动神经元中水平最高。NGFR mRNA也在许多间充质细胞群体中短暂表达，包括鳃弓、体节、肌肉原基和毛囊中的细胞。讨论了神经生长因子受体广泛胚胎表达的功能意义。
