Li Aiwen, Qiu Jiguo, Chen Dongzhi, Ye Jiexu, Wang Yuhong, Tong Lu, Jiang Jiandong, Chen Jianmeng
College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Mar Drugs. 2017 May 31;15(6):156. doi: 10.3390/md15060156.
The presence of nicotine and nicotinic acid (NA) in the marine environment has caused great harm to human health and the natural environment. Therefore, there is an urgent need to use efficient and economical methods to remove such pollutants from the environment. In this study, a nicotine and NA-degrading bacterium-strain JQ581-was isolated from sediment from the East China Sea and identified as a member of based on morphology, physio-biochemical characteristics, and 16S rDNA gene analysis. The relationship between growth and nicotine/NA degradation suggested that strain JQ581 was a good candidate for applications in the bioaugmentation treatment of nicotine/NA contamination. The degradation intermediates of nicotine are pseudooxynicotine (PN) and 3-succinoyl-pyridine (SP) based on UV, high performance liquid chromatography, and liquid chromatography-mass spectrometry analyses. However, 6-hydroxy-3-succinoyl-pyridine (HSP) was not detected. NA degradation intermediates were identified as 6-hydroxynicotinic acid (6HNA). The whole genome of strain JQ581 was sequenced and analyzed. Genome sequence analysis revealed that strain JQ581 contained the gene clusters for nicotine and NA degradation. This is the first report where a marine-derived strain had the ability to degrade nicotine and NA simultaneously.
海洋环境中尼古丁和烟酸（NA）的存在已对人类健康和自然环境造成了极大危害。因此，迫切需要采用高效且经济的方法从环境中去除此类污染物。在本研究中，从中国东海沉积物中分离出一株尼古丁和NA降解菌——菌株JQ581，并基于形态学、生理生化特征及16S rDNA基因分析将其鉴定为某属的成员。生长与尼古丁/NA降解之间的关系表明，菌株JQ581是尼古丁/NA污染生物强化处理应用的良好候选菌株。基于紫外、高效液相色谱和液相色谱-质谱分析，尼古丁的降解中间体为假氧化尼古丁（PN）和3-琥珀酰吡啶（SP）。然而，未检测到6-羟基-3-琥珀酰吡啶（HSP）。NA降解中间体被鉴定为6-羟基烟酸（6HNA）。对菌株JQ581的全基因组进行了测序和分析。基因组序列分析表明，菌株JQ581含有尼古丁和NA降解的基因簇。这是首次报道海洋来源的菌株具有同时降解尼古丁和NA的能力。
