Lougheed Stephen C, Gibbs H Lisle, Prior Kent A, Weatherhead Patrick J
Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada.
Evolution. 1999 Dec;53(6):1995-2001. doi: 10.1111/j.1558-5646.1999.tb04581.x.
We investigated the distribution of variation at six microsatellite loci in the black rat snake (Elaphe obsoleta obsoleta). Sampling occurred at three hierarchical scales ranging from communal hibernacula to regional populations, with most locales situated within the Frontenac Axis region of eastern Ontario. We detected no statistically significant pairwise differentiation (F and R ) between hibernacula within the same subpopulations (interhibernaculum distance <6 km). However, isolation-by-distance was evident among locales within the Frontenac Axis (maximum of 50 km) and among regional populations (maximum of 1500 km). Conservative estimates of N derived from heterozygosity values ranged from approximately 600 to 2000. These values suggest relatively large genetic neighborhoods encompassing many communal hibernacula. Our results considered together suggest viscosity of gene flow over relatively short distances (tens of kilometers), but substantial genetic exchange among local hibernacula.
我们研究了黑鼠蛇（Elaphe obsoleta obsoleta）六个微卫星位点的变异分布情况。采样在从群落冬眠场所到区域种群的三个层次尺度上进行，大多数地点位于安大略省东部的弗龙特纳克轴地区。我们发现在同一亚种群内的冬眠场所之间（冬眠场所间距离<6公里），没有统计学上显著的成对分化（F和R）。然而，在弗龙特纳克轴内的地点之间（最大距离50公里）以及区域种群之间（最大距离1500公里），距离隔离是明显的。根据杂合度值得出的N的保守估计范围约为600至2000。这些值表明包含许多群落冬眠场所的相对较大的遗传邻域。综合我们的结果表明，在相对较短的距离（几十公里）内基因流具有粘性，但当地冬眠场所之间存在大量的基因交换。
