Haynie Michelle L, Fulhorst Charles F, Rood Michael, Bennett Stephen G, Hess Barry D, Bradley Robert D
Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409-3131, USA (MLH, RDB).
J Mammal. 2007 Jun 1;88(3):745-758. doi: 10.1644/06-MAMM-A-041R1.1.
Five microsatellite loci were used to develop multilocus genotypes for Neotoma macrotis (n = 128) and N. fuscipes (n = 29). Several statistical analyses were used to estimate genetic structure, levels of genetic variability, and degree of relatedness within groups of these 2 species. Samples of N. macrotis represented 2 groups and 4 population clusters throughout southern California. Samples of N. fuscipes represented 2 regions in northern and southern California. Genetic structure was detected among samples of N. macrotis and N. fuscipes at a regional level. Both species displayed moderate to high genetic diversity in terms of mean expected heterozygosity (0.939 and 0.804 for N. macrotis and N. fuscipes, respectively) and mean polymorphic information content (0.930 and 0.761 for N. macrotis and N. fuscipes, respectively). Mean relatedness values within regions and populations of N. macrotis indicated 4th-order levels of relatedness within groups (e.g., distant-cousin relationships). Mean relatedness values within regions of N. fuscipes indicated 2nd-order (e.g., half-sibling) relationships within the northern region and 3rd-order (e.g., cousin) relationships in the southern region. One locus in particular (Nma04) was determined to be diagnostic in distinguishing between these 2 species.
利用五个微卫星位点构建了大耳林鼠（n = 128）和褐林鼠（n = 29）的多位点基因型。运用多种统计分析方法来估计这两个物种群体内的遗传结构、遗传变异水平以及亲缘关系程度。大耳林鼠的样本代表了南加州的2个群体和4个种群聚类。褐林鼠的样本代表了加州北部和南部的2个区域。在区域水平上，大耳林鼠和褐林鼠的样本之间检测到了遗传结构。就平均期望杂合度而言（大耳林鼠和褐林鼠分别为0.939和0.804）以及平均多态信息含量而言（大耳林鼠和褐林鼠分别为0.930和0.761），这两个物种均表现出中度到高度的遗传多样性。大耳林鼠区域和种群内的平均亲缘关系值表明群体内存在四阶亲缘关系水平（例如，远亲关系）。褐林鼠区域内的平均亲缘关系值表明北部区域内存在二阶（例如，半同胞）关系，而南部区域内存在三阶（例如，堂亲）关系。确定了一个特别的位点（Nma04）可用于区分这两个物种。
