Zhang H H, He J, Bao X J, Hu X, Wang M, Zhang J, Wu X J
Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Zhonghua Xue Ye Xue Za Zhi. 2017 May 14;38(5):421-426. doi: 10.3760/cma.j.issn.0253-2727.2017.05.013.
To analyze the distribution and proportion of donor-specific activated killer cell immunoglobulin like receptor (aKIR) genes and their clinical application values in unrelated allogeneic hematopoietic stem cell transplantation (allo-HSCT) . Retrospective analyses of KIR genotyping using polymerase chain reaction with sequence specific primers (PCR-SSP) were performed in 216 pairs of donors and recipients. The frequency of donor-specific KIR genes was 53.7% (116/216) in 216 patients receiving unrelated allo-HSCT, with the frequency of 78.3% (112/143) in the KIR genes mismatched group and 5.5% (4/73) in matched group. Of the 116 patients with detectable donor-specific KIR genes, 99.1% (115/116) patients had various donor-specific aKIR genes. Among 55 pairs of donors' KIR-Bx genotype and patients' KIR-AA genotype group, the most commonly observed genotypes were Bx1, Bx2, Bx3, Bx4, in which the donor-specific KIR genes were respectively KIR 3DS1, 2DL5A, 2DS5, 2DS1; KIR 3DS1, 2DL5A, 2DS3, 2DS1; KIR 2DS2, 2DL2; KIR 2DS2, 2DL2, 3DS1, 2DL5A, 2DS5, 2DS1. Of 44 pairs of donors' KIR-AA genotype and patients' KIR-Bx (AB) genotype group, 36.4% (16/44) recipients had donor-specific KIR2DS4 (FUL) gene. In 143 pairs of KIR mismatched group, the frequencies of donor-specific KIR genes were KIR2DS1 (35.7%) , KIR3DS1 (32.9%) , KIR2DS5 (29.4%) , KIR2DS4 (FUL) (25.9%) , KIR2DL2 (25.2%) , KIR2DS2 (24.5%) , KIR2DS3 (21.7%) and KIR3DL1 (8.4%) , respectively. The donor-specific aKIR genes mainly existed in KIR mismatched group after unrelated allo-HSCT, and the different pairs of donors' and patients' KIR genotypes led to the diverse donor-specific aKIR. But there were higher specific aKIR genes in higher frequency of KIR AA, Bx1, Bx2, Bx3, Bx4 genotypes. All these can provide the experimental basis for studying the role of the donor-specific aKIR genes on the prognosis of HSCT.
分析供者特异性活化杀伤细胞免疫球蛋白样受体（aKIR）基因在非亲缘异基因造血干细胞移植（allo-HSCT）中的分布、比例及其临床应用价值。采用序列特异性引物聚合酶链反应（PCR-SSP）对216对供受者进行KIR基因分型回顾性分析。216例接受非亲缘allo-HSCT患者中，供者特异性KIR基因频率为53.7%（116/216），其中KIR基因不匹配组频率为78.3%（112/143），匹配组频率为5.5%（4/73）。116例可检测到供者特异性KIR基因的患者中，99.1%（115/116）患者有不同的供者特异性aKIR基因。在55对供者KIR-Bx基因型与患者KIR-AA基因型组中，最常见的基因型为Bx1、Bx2、Bx3、Bx4，其中供者特异性KIR基因分别为KIR 3DS1、2DL5A、2DS5、2DS1；KIR 3DS1、2DL5A、2DS3、2DS1；KIR 2DS2、2DL2；KIR 2DS2、2DL2、3DS1、2DL5A、2DS5、2DS1。44对供者KIR-AA基因型与患者KIR-Bx（AB）基因型组中，36.4%（16/44）受者有供者特异性KIR2DS4（FUL）基因。143对KIR不匹配组中，供者特异性KIR基因频率分别为KIR2DS1（35.7%）、KIR3DS1（32.9%）、KIR2DS5（29.4%）、KIR2DS4（FUL）（25.9%）、KIR2DL2（25.2%）、KIR2DS2（24.5%）、KIR2DS3（21.7%）和KIR3DL1（8.4%）。非亲缘allo-HSCT后供者特异性aKIR基因主要存在于KIR不匹配组，不同供受者KIR基因型组合导致供者特异性aKIR各异。但KIR AA、Bx1、Bx2、Bx3、Bx4基因型频率较高时，特异性aKIR基因也较高。这些均可为研究供者特异性aKIR基因对HSCT预后的作用提供实验依据。
