Qing Liming, Lei Pengfei, Tang Juyu, Wu Pangfeng, Wang Long, Xie Jie, Hu Yihe
Department of Orthopaedics, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
Exp Ther Med. 2017 May;13(5):2012-2018. doi: 10.3892/etm.2017.4205. Epub 2017 Mar 8.
Ischemic necrosis of the surgical flap is a common complication. The behavior of choke vessels has an important role in skin flap survival. However, the mechanism of choke vessel remodeling has remained elusive. The purpose of the present study was to investigate the possible association between inflammatory responses and choke vessel remodeling in the extended perforator flap model in rats. After flap elevation, the animals were randomly divided into three groups (n=6 in each) for tissue analysis at three, five or seven days after flap surgery. Six additional rats served as a control group (no flap elevation). Tissue samples were collected from the choke zone for histological, western blot and PCR analyses. Monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α (TNF-α) as inflammatory cytokines were examined in the present study. Histopathological analysis showed that dilation of choke vessels and increased vessel wall thickness was obvious after flap elevation. It also showed edema, inflammation cell aggregation after the operation. Compared with the control group, the protein and mRNA expression levels of MCP-1 and TNF-α were significantly increased at days 3, 5 and 7 after flap elevation, while reaching a maximum at day 5. These findings indicated that inflammatory responses may have an important role in choke vessel remodeling. MCP-1 and TNF-α may be considered as potential targets for modulating the behavior of choke vessels.
手术皮瓣缺血性坏死是一种常见的并发症。吻合血管的行为在皮瓣存活中起着重要作用。然而，吻合血管重塑的机制仍不清楚。本研究的目的是探讨大鼠穿支皮瓣模型中炎症反应与吻合血管重塑之间可能存在的关联。皮瓣掀起后，将动物随机分为三组（每组n = 6），分别在皮瓣手术后3天、5天或7天进行组织分析。另外6只大鼠作为对照组（未掀起皮瓣）。从吻合血管区采集组织样本进行组织学、蛋白质印迹和PCR分析。本研究检测了作为炎症细胞因子的单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-α（TNF-α）。组织病理学分析显示，皮瓣掀起后吻合血管扩张和血管壁厚度增加明显。还显示术后有水肿、炎症细胞聚集。与对照组相比，皮瓣掀起后第3天、5天和7天MCP-1和TNF-α的蛋白质和mRNA表达水平显著升高，在第5天达到最高。这些结果表明，炎症反应可能在吻合血管重塑中起重要作用。MCP-1和TNF-α可被视为调节吻合血管行为的潜在靶点。
