Xie Tiancheng, Hu Guanghui, Dong Binbin, Yan Yangye, Liu Min, Yao Xudong, Zheng Junhua, Xu Yunfei
Department of Urology, Shanghai Tenth People's Hospital, Tongji University, School of Medicine, Shanghai 200072, P.R. China.
Exp Ther Med. 2017 May;13(5):2169-2176. doi: 10.3892/etm.2017.4239. Epub 2017 Mar 20.
Roscovitine is a cyclin-dependent kinase inhibitor, which has been previously investigated for its anticancer effects. It has also been confirmed that roscovitine can downregulate the expression of myeloid cell leukemia-1 protein to inhibit inflammation. In the present study, roscovitine was used to treat inflammation in lipopolysaccharide (LPS)-induced model mice. At the cellular level, Leydig cells isolated from mouse testis were assessed for inflammatory factors. It was revealed that roscovitine successfully reduced inflammation-associated injury induced by LPS pretreatment. At the molecular level, roscovitine was found to exert this effect through promotion of adenosine monophosphate-activated protein kinase phosphorylation. To the best of our knowledge, the present study was the first to suggest that roscovitine has a protective role in Leydig cells through its anti-inflammatory action.
罗斯考维汀是一种细胞周期蛋白依赖性激酶抑制剂，此前已对其抗癌作用进行过研究。也已证实罗斯考维汀可下调髓样细胞白血病-1蛋白的表达以抑制炎症。在本研究中，罗斯考维汀被用于治疗脂多糖（LPS）诱导的模型小鼠的炎症。在细胞水平上，对从小鼠睾丸分离出的睾丸间质细胞的炎症因子进行了评估。结果显示，罗斯考维汀成功减轻了LPS预处理诱导的炎症相关损伤。在分子水平上，发现罗斯考维汀通过促进腺苷单磷酸激活的蛋白激酶磷酸化发挥这种作用。据我们所知，本研究首次表明罗斯考维汀通过其抗炎作用对睾丸间质细胞具有保护作用。
