Tang Ren-Yi, Wang Zun, Chen Hong-Qi, Zhu Si-Bo
Shanghai Starriver Bilingual School, 2588 Jindu Road, Minhang District, Shanghai 201108, China.
Department of General Surgery, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Biomed Res Int. 2017;2017:1038984. doi: 10.1155/2017/1038984. Epub 2017 Apr 16.
To demonstrate the regulatory role of miRNA in colorectal carcinoma (CRC) and reveal the transcript markers that may be associated with CRC clinical outcomes. Herein, we analyzed both mRNA and miRNA gene expression profiles of 255 CRC tumor samples from The Cancer Genome Atlas project to reveal the regulatory association between miRNA and mRNA. Also, the potential role of gene coexpression network in CRC has been explored. The negative correlation between miR-200c and DCN (Decorin) was calculated in CRC, indicating that DCN could be a potential target of miR-200c. Clinical features indicated that colon polyp history and overall survival were significantly related to the expression level of miR-200c. Three coexpression networks have been constructed, and genes involved in the networks are related to cell cycle, NOTCH, and mTOR signaling pathways. . Our result provides a new insight into cancer related mRNA coexpression network in CRC research.
为了证明微小RNA（miRNA）在结直肠癌（CRC）中的调控作用，并揭示可能与CRC临床结果相关的转录标志物。在此，我们分析了来自癌症基因组图谱项目的255份CRC肿瘤样本的mRNA和miRNA基因表达谱，以揭示miRNA与mRNA之间的调控关联。此外，还探讨了基因共表达网络在CRC中的潜在作用。在CRC中计算了miR-200c与DCN（核心蛋白聚糖）之间的负相关性，表明DCN可能是miR-200c的潜在靶点。临床特征表明，结肠息肉病史和总生存期与miR-200c的表达水平显著相关。构建了三个共表达网络，网络中涉及的基因与细胞周期、NOTCH和mTOR信号通路相关。我们的结果为CRC研究中癌症相关的mRNA共表达网络提供了新的见解。
