Yu Changning, Li Yanjiao, Zhang Bolin, Lin Meng, Li Jiaolong, Zhang Lin, Wang Tianjiao, Gao Feng, Zhou Guanghong
Synergetic Innovation Center of Food Safety and Nutrition, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University , No. 1 Weigang Road, Nanjing, 210095 Jiangsu China.
J Agric Food Chem. 2016 Feb 24;64(7):1557-64. doi: 10.1021/acs.jafc.5b06089. Epub 2016 Feb 15.
Three iso-energetic and iso-nitrogenous diets were fed to finishing pigs for 28 days to investigate the mammalian target of rapamycin (mTOR) and ubiquitin-proteasome signaling pathways of skeletal muscle by altering compositions of dietary energy sources. Diet A, diet B, and diet C contained 44.1%, 37.6%, and 30.9% starch; 5.9%, 9.5%, and 14.3% ether extract (EE); and 12.6%, 15.4%, and 17.8% neutral detergent fiber (NDF), respectively. An increase of mRNA expression of MuRF1 (1.09 ± 0.10 vs 1.00 ± 0.08) and MAFbx (1.10 ± 0.06 vs 1.00 ± 0.11) and a decrease of concentrations of plasma insulin (8.2 ± 0.8 vs 10.8 ± 1.2) and glucose (5.76 ± 0.12 vs 6.43 ± 0.33) together with mRNA expression of IRS (0.78 ± 0.19 vs 1.01 ± 0.05) and Akt (0.92 ± 0.01 vs 1.00 ± 0.05) were observed in pigs fed diet C compared with diet A. Protein levels of total and phosphorylated mTOR (0.31 ± 0.04 vs 0.48 ± 0.03 and 0.39 ± 0.01 vs 0.56 ± 0.02), 4EBP1 (0.66 ± 0.06 vs 0.90 ± 0.09 and 0.60 ± 0.12 vs 0.84 ± 0.09), and S6K1 (0.66 ± 0.01 vs 0.89 ± 0.01 and 0.48 ± 0.03 vs 0.79 ± 0.02) were decreased; however, total and phosphorylated AMPK (0.96 ± 0.06 vs 0.64 ± 0.04 and 0.97 ± 0.09 vs 0.61 ± 0.09) were increased in pigs fed diet C compared with diet A. In conclusion, diet C suppressed the mTOR pathway and accelerated the ubiquitin-proteasome pathway in skeletal muscle of finishing pigs.
给育肥猪饲喂三种等能量和等氮的日粮28天，通过改变日粮能量来源的组成来研究骨骼肌中雷帕霉素靶蛋白（mTOR）和泛素-蛋白酶体信号通路。日粮A、日粮B和日粮C分别含有44.1%、37.6%和30.9%的淀粉；5.9%、9.5%和14.3%的乙醚提取物（EE）；以及12.6%、15.4%和17.8%的中性洗涤纤维（NDF）。与日粮A相比，饲喂日粮C的猪中，MuRF1的mRNA表达增加（1.09±0.10对1.00±0.08），MAFbx的mRNA表达增加（1.10±0.06对1.00±0.11），血浆胰岛素浓度降低（8.2±0.8对10.8±1.2），葡萄糖浓度降低（5.76±0.12对6.43±0.33），同时IRS的mRNA表达降低（0.78±0.19对1.01±0.05），Akt的mRNA表达降低（0.92±0.01对1.00±0.0该文档为医学专业学术文献翻译，仅供参考，具体翻译内容可能需要根据上下文和专业知识进行调整。5）。与日粮A相比，饲喂日粮C的猪中，总mTOR和磷酸化mTOR的蛋白水平降低（0.31±0.04对0.48±0.03和0.39±0.01对0.56±0.02），4EBP1的蛋白水平降低（0.66±0.06对0.90±0.09和0.60±0.12对0.84±0.09），S6K1的蛋白水平降低（0.66±0.01对0.89±0.01和0.48±0.03对0.79±0.02）；然而，总AMPK和磷酸化AMPK的蛋白水平升高（0.96±0.06对0.64±0.04和0.97±0.09对0.61±0.09）。总之，日粮C抑制了育肥猪骨骼肌中的mTOR通路并加速了泛素-蛋白酶体通路。
