Raaz-Schrauder Dorette, Schrauder Michael G, Stumpf Christian, Lewczuk Piotr, Kilian Tobias, Dietel Barbara, Garlichs Christoph D, Schlundt Christian, Achenbach Stephan, Klinghammer Lutz
Friedrich-Alexander University Erlangen-Nürnberg (FAU), Department of Cardiology and Angiology, Erlangen University Hospital, Ulmenweg 18, 91054, Erlangen, Germany.
Friedrich-Alexander University Erlangen-Nürnberg (FAU), Department of Obstetrics and Gynecology, Erlangen University Hospital, Comprehensive Cancer Center Erlangen-EMN, Erlangen, Germany.
Heart Vessels. 2017 Nov;32(11):1304-1313. doi: 10.1007/s00380-017-0998-z. Epub 2017 May 31.
Osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) are regulators of bone remodeling, but are also considered to play important roles in coronary artery disease (CAD). This study evaluated potential associations of soluble (s) RANKL and OPG with atherosclerosis-relevant cytokines. Blood was collected from 414 individuals who presented to our hospital with intermediate likelihood for CAD for further examination. Plasma concentrations of total sRANKL, OPG, and 20 cytokines were measured using sandwich-type enzyme-linked immunoassays (ELISAs; OPG and sRANKL) and Luminex laser-based fluorescence analysis and correlated with each other. The plasma levels of interferon-γ (IFN-γ) and the T-helper cell 2 cytokines interleukin-4 (IL-4) and IL-13 showed a positive correlation with sRANKL. The association with sRANKL levels was negative for IFN-γ-induced protein-10 (IP-10) and monocyte chemotactic protein-1 (MCP-1). The strongest independent association with sRANKL in multivariable analyses was found for IFN-γ (positive) and IP-10 (negative), while IL-13 showed a positive and independent association with OPG plasma levels. OPG and sRANKL plasma levels correlate strongly and independently with specific circulating atherosclerosis-related cytokines in patients with intermediate cardiovascular risk.
骨保护素（OPG）和核因子κB受体激活剂配体（RANKL）是骨重塑的调节因子，但也被认为在冠状动脉疾病（CAD）中起重要作用。本研究评估了可溶性（s）RANKL和OPG与动脉粥样硬化相关细胞因子之间的潜在关联。收集了414名因CAD可能性中等而前来我院就诊的患者的血液进行进一步检查。使用夹心型酶联免疫吸附测定法（ELISA；用于OPG和sRANKL）和基于Luminex激光的荧光分析法测量了总sRANKL、OPG和20种细胞因子的血浆浓度，并对它们进行相互关联分析。干扰素-γ（IFN-γ）以及辅助性T细胞2细胞因子白细胞介素-4（IL-4）和IL-13的血浆水平与sRANKL呈正相关。IFN-γ诱导蛋白10（IP-10）和单核细胞趋化蛋白1（MCP-1）与sRANKL水平呈负相关。在多变量分析中，与sRANKL最强的独立关联在IFN-γ（正相关）和IP-10（负相关）中被发现，而IL-13与OPG血浆水平呈正相关且独立相关。在心血管风险中等的患者中，OPG和sRANKL血浆水平与特定的循环动脉粥样硬化相关细胞因子密切且独立相关。
