Shinohara Hidefumi, Matsubayashi Yoshikatsu
Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya, 464-8602, Japan.
Methods Mol Biol. 2017;1621:59-68. doi: 10.1007/978-1-4939-7063-6_6.
Defining the ligand-binding activity of receptors is important because the binding of ligands is the initial reaction in secreted ligand-dependent cell-to-cell communication. Photoaffinity labeling is one of the most efficient biochemical techniques for detecting direct ligand-receptor interactions. Here, we describe photoaffinity labeling to visualize the direct interaction between peptide ligands and their receptors by using photoactivatable and radioactive peptide ligand derivatives.
定义受体的配体结合活性很重要，因为配体结合是分泌型配体依赖性细胞间通讯中的初始反应。光亲和标记是检测直接配体-受体相互作用最有效的生化技术之一。在此，我们描述了通过使用光可激活的放射性肽配体衍生物进行光亲和标记，以可视化肽配体与其受体之间的直接相互作用。
