Tokyo University of Agriculture and Technology, Department of Biotechnology and Life Science, Koganei-shi, Tokyo 184-8588, Japan.
Org Biomol Chem. 2021 Feb 18;19(6):1268-1273. doi: 10.1039/d0ob01688h.
Rapid access to appropriately functionalized probes is crucial in chemical labeling approaches to target identification studies. We designed and synthesized clickable gold-nanoparticles as generic probe precursors that enable (1) one-step ligand derivatization by click chemistry, and (2) facile photoaffinity labeling application. Using cholesterol as a model ligand, we successfully demonstrated the utility of the ligand-clicked probe in photoaffinity labeling of endogenously expressed oxysterol-binding protein (OSBP) in cell lysate.
快速获得功能化探针在目标识别研究的化学标记方法中至关重要。我们设计并合成了可点击的金纳米颗粒作为通用探针前体，能够 (1) 通过点击化学进行一步配体衍生化，以及 (2) 方便地进行光亲和标记应用。我们使用胆固醇作为模型配体，成功地证明了配体点击探针在细胞裂解物中内源表达的氧化固醇结合蛋白 (OSBP) 的光亲和标记中的应用。
