Stimpel M, Neyses L, Locher R, Knorr M, Vetter W
Department of Internal Medicine, University Hospital Zurich, Switzerland.
J Hypertens Suppl. 1985 Dec;3(3):S49-51.
It has recently been shown that human red blood cells possess a voltage-independent calcium channel which can be influenced by in vitro modification of the membraneous cholesterol content. To determine whether there is also a link between plasma lipids and the calcium influx through this channel under in vivo conditions, the calcium influx was measured in red blood cells from 51 male donors (aged 41 +/- 5 years). The influx through the channel was defined as the nitrendipine (15 mumol/l)-inhibitable part of 45Ca2+ influx. The Ca(2+)-ejecting ATPase was inhibited by vanadate. The results demonstrate a strong inverse relationship (r = -0.81; P < 0.001) between the plasma concentration of high density lipoproteins (HDL) and 45Ca2+ influx. No significant correlation was found between 45Ca2+ influx and triglycerides, low density lipoproteins (LDL), very low density lipoproteins (VLDL), total plasma cholesterol or extracellular electrolytes (K+, Na+, Ca2+, Mg2+). The results indicate that HDL are involved in the modulation of the calcium channel and provide a link between the cellular cholesterol turnover and the calcium influx in the pathogenesis of atherosclerosis and hypertension.
最近研究表明，人类红细胞拥有一种电压非依赖性钙通道，该通道可受膜胆固醇含量的体外修饰影响。为了确定在体内条件下血浆脂质与通过该通道的钙内流之间是否也存在联系，对51名男性献血者（年龄41±5岁）的红细胞中的钙内流进行了测量。通过该通道的内流被定义为尼群地平（15μmol/l）可抑制的45Ca2+内流部分。钒酸盐抑制了Ca(2+) - 排出ATP酶。结果显示，高密度脂蛋白（HDL）的血浆浓度与45Ca2+内流之间存在强烈的负相关关系（r = -0.81；P < 0.001）。未发现45Ca2+内流与甘油三酯、低密度脂蛋白（LDL）、极低密度脂蛋白（VLDL）、总血浆胆固醇或细胞外电解质（K+、Na+、Ca2+、Mg2+）之间存在显著相关性。结果表明，HDL参与了钙通道的调节，并在动脉粥样硬化和高血压的发病机制中提供了细胞胆固醇代谢与钙内流之间的联系。
