Bensch Staffan, Hasselquist Dennis, von Schantz Torbjörn
Department of Ecology, Animal Ecology, University of Lund, Ecology Building, S-223 62 Lund, Sweden.
Molecular Population Biology, Wallenberg Laboratory, University of Lund, Box 7031, S-220 07 Lund, Sweden.
Evolution. 1994 Apr;48(2):317-326. doi: 10.1111/j.1558-5646.1994.tb01314.x.
The DNA-fingerprinting technique was used to find the true pedigrees and to detect the overall genetic similarity between mates of great reed warblers (Acrocephalus arundinaceus) at an isolated breeding site in Sweden. The study covered 4 yr preceded by 3 yr when almost all adults and nestlings in the study area had been banded. DNA fingerprinting revealed that the putative father had sired 97% of the young (N = 455). The mate's genetic similarity, revealed as the proportion of bands shared in restriction fragment length polymorphism (RFLP) patterns, was high compared with other species of wild birds. Also, band sharing was higher between mates native to the area than between pairs in which the female was experimentally introduced from a distant breeding site. Hatching success of eggs was negatively correlated with the degree of genetic similarity between the mates, whereas pedigree data, up to the level of great-grandparents, clearly demonstrated an absence of close inbreeding. These are the first data showing a significant fitness cost associated with the choice of a mate that has high genetic similarity, even if it is not a close kin. This cost might be caused by generalized negative consequences of genomewide inbreeding in the present study, possibly accentuated by recent population bottlenecks.
采用DNA指纹技术来确定真实的谱系，并检测瑞典一个孤立繁殖地大苇莺（Acrocephalus arundinaceus）配偶之间的总体遗传相似性。该研究涵盖了4年，在此之前的3年里，研究区域内几乎所有成年个体和雏鸟都已佩戴环志。DNA指纹分析显示，推定的父亲生育了97%的幼鸟（N = 455）。配偶之间的遗传相似性，以限制性片段长度多态性（RFLP）模式中共享条带的比例来表示，与其他野生鸟类相比很高。此外，本地配偶之间的条带共享率高于雌性从远处繁殖地实验性引入的配对。卵的孵化成功率与配偶之间的遗传相似程度呈负相关，而一直追溯到曾祖父母水平的谱系数据清楚地表明不存在近亲繁殖。这些是首批数据，表明选择遗传相似性高的配偶（即使不是近亲）会带来显著的适合度代价。这种代价可能是由本研究中全基因组近亲繁殖的普遍负面后果导致的，近期的种群瓶颈可能使其更加严重。
