Vyas D K, Moritz C, Peccinini-Seale D, Wright J W, Brown W M
Laboratory of Molecular Systematics, Museum of Zoology and Department of Biology, University of Michigan, Ann Arbor, MI, 48109-1048, USA.
Departamento de Biologia, Instituto de Biociencias, Universidade de Sao Paulo, 05499, Sao Paulo, BRAZIL.
Evolution. 1990 Jul;44(4):922-932. doi: 10.1111/j.1558-5646.1990.tb03814.x.
Restriction endonuclease analyses were performed on mitochondrial DNAs (mtDNAs) representing unisexual parthenogenetic (cytotypes A, B, and C) and bisexual (cytotypes D and E) populations of Amazonian lizards presently regarded as Cnemidophorus lemniscatus. The results of mtDNA cleavage map comparisons among these C. lemniscatus indicated that (1) there was no cleavage site variation among the unisexuals, (2) mtDNAs from the bisexual cytotypes D and E differed in sequence from one another by about 13%, and (3) mtDNAs from cytotypes A-C differed from those of cytotype D by about 5% and from those of cytotype E by about 13%. Higher resolution restriction fragment size comparisons confirmed the high degree of similarity among the unisexual mtDNAs, but identified 12 cleavage site variants among the 13 cytotype D mtDNAs examined. Both cladistic and phenetic (UPGMA) analyses of the data indicate that the unisexual and cytotype D mtDNAs form a single clade, suggesting that a female of cytotype D was the maternal progenitor of the unisexuals. The similarity among the unisexual mtDNAs and the variability among those of cytotype D suggest that the three unisexual cytotypes arose recently from a common maternal lineage. The mtDNA variability observed among cytotype D individuals has a strong geographic component, suggesting that the unisexuals arose from one or a few geographically proximal populations. The mtDNA comparisons also support the conclusion, based on allozyme comparisons (Sites et al., 1990, this issue), that cytotypes D and E, although presently allocated to C. lemniscatus, are separate species.
对目前被视为丽斑麻蜥（Cnemidophorus lemniscatus）的亚马逊蜥蜴的单性孤雌生殖（细胞型A、B和C）及两性生殖（细胞型D和E）群体的线粒体DNA（mtDNA）进行了限制性内切酶分析。这些丽斑麻蜥之间的mtDNA切割图谱比较结果表明：（1）单性生殖群体之间没有切割位点变异；（2）两性细胞型D和E的mtDNA序列彼此相差约13%；（3）细胞型A - C的mtDNA与细胞型D的相差约5%，与细胞型E的相差约13%。更高分辨率的限制性片段大小比较证实了单性生殖mtDNA之间的高度相似性，但在所检测的13个细胞型D的mtDNA中鉴定出了12个切割位点变异体。对数据进行的分支分析和表型分析（UPGMA）均表明，单性生殖和细胞型D的mtDNA形成一个单一支系，这表明细胞型D的一只雌性是单性生殖群体的母系祖先。单性生殖mtDNA之间的相似性以及细胞型D的mtDNA之间的变异性表明，这三个单性生殖细胞型最近起源于一个共同的母系谱系。在细胞型D个体中观察到的mtDNA变异性具有很强的地理成分，这表明单性生殖群体起源于一个或几个地理上相邻的群体。mtDNA比较也支持了基于等位酶比较（Sites等人，1990年，本期）得出的结论，即细胞型D和E虽然目前被归为丽斑麻蜥，但它们是独立的物种。
