Gupta Samir, Ray Krishanu
Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400 005, India.
J Biosci. 2017 Jun;42(2):285-297. doi: 10.1007/s12038-017-9678-5.
Spermatogenesis, involving multiple transit amplification divisions and meiosis, occurs within an enclosure formed by two somatic cells. As the cohort of germline cells divide and grow, the surface areas of the somatic cells expand maintaining a tight encapsulation throughout the developmental period. Correlation between the somatic cell growth and germline development is unclear. Here, we report standardization of a quantitative assay developed for estimating the somatic roles of target molecules on germline division and differentiation in Drosophila testis. Using the assay, we studied the somatic roles of phosphatidylinositol-3-kinase (PI3K). It revealed that the expression of PI3KDN is likely to facilitate the early germline development at all stages, and an increase in the somatic PI3K activity during the early stages delays the transition to spermatocyte stage. Together, these results suggest that somatic cell growth plays an important role in regulating the rate of germline development.
精子发生过程涉及多个过渡增殖分裂和减数分裂，发生在由两个体细胞形成的封闭结构内。随着生殖系细胞群体的分裂和生长，体细胞的表面积不断扩大，在整个发育过程中保持紧密包裹。体细胞生长与生殖系发育之间的相关性尚不清楚。在这里，我们报告了一种定量分析方法的标准化，该方法用于评估果蝇睾丸中靶分子对生殖系分裂和分化的体细胞作用。使用该分析方法，我们研究了磷脂酰肌醇-3-激酶（PI3K）的体细胞作用。结果表明，PI3KDN的表达可能在所有阶段促进生殖系早期发育，早期体细胞PI3K活性的增加会延迟向精母细胞阶段的转变。总之，这些结果表明体细胞生长在调节生殖系发育速率中起重要作用。
