Feng C, Li Y, Spitale R C
Department of Pharmaceutical Sciences, University of California, Irvine, 2403 Natural Sciences I, Irvine CA 92617, USA.
Org Biomol Chem. 2017 Jun 21;15(24):5117-5120. doi: 10.1039/c7ob01009e.
Elucidating gene expression programs within a cell-specific manner is a grand challenge for biologists. Harder still is the ability to have kinetic control over such experiments. Metabolic labeling with bioorthogonally-functionalized metabolic intermediates provides a means to profile RNA expression in a cell-specific manner, but there is still a lack of kinetic resolution. Herein we present the synthesis and evaluation of photocaged metabolic uracil intermediates. We compare the photo-decaging properties and demonstrate their utility in metabolic labeling experiments in a cell-specific manner. We anticipate that our approach will have far-reaching impact as it provides control over tagging of nascent RNA.
以细胞特异性方式阐明基因表达程序是生物学家面临的一项重大挑战。更具挑战性的是对这类实验进行动力学控制的能力。用生物正交功能化代谢中间体进行代谢标记提供了一种以细胞特异性方式分析RNA表达的方法，但仍然缺乏动力学分辨率。在此，我们展示了光笼代谢尿嘧啶中间体的合成和评估。我们比较了光解笼特性，并证明了它们在细胞特异性代谢标记实验中的效用。我们预计，我们的方法将产生深远影响，因为它能够控制新生RNA的标记。
