Liao Xianjiu, Li Zhenzhong, Peng Tingting, Li Jie, Qin Fengying, Huang Zuliang
School of Pharmacy, Youjiang Medical University for Nationalities, Baise, Guangxi, People's Republic of China.
School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, Guangxi, People's Republic of China.
Luminescence. 2017 Dec;32(8):1411-1416. doi: 10.1002/bio.3338. Epub 2017 Jun 1.
A novel ultra-sensitive fluorescent sensor for monitoring microRNA (miRNA) in living cells was constructed by utilizing a hybridization chain reaction (HCR) as the signal amplification with a carbon nitride nanosheet (CNNS) as a carrier. The Cy5-labeled hairpin DNA could be adsorbed onto the surface of CNNS, resulting in fluorescence quenching of Cy5. When treated with complementary miRNA, the fluorescence was recovered because miRNA could efficiently trigger an HCR, which led to the release of the HCR products from the CNNS. This intracellular HCR strategy can be used for ultra-sensitive monitoring of intracellular miRNA. The main advantages of the proposed method are its simplicity, high sensitivity, high specificity and low toxicity for monitoring low-level biomarkers.
通过利用杂交链式反应（HCR）作为信号放大手段，并以氮化碳纳米片（CNNS）作为载体，构建了一种用于监测活细胞中微小RNA（miRNA）的新型超灵敏荧光传感器。Cy5标记的发夹DNA可吸附在CNNS表面，导致Cy5荧光猝灭。当用互补miRNA处理时，荧光恢复，因为miRNA可有效触发HCR，从而导致HCR产物从CNNS上释放。这种细胞内HCR策略可用于超灵敏监测细胞内miRNA。该方法的主要优点在于其简单性、高灵敏度、高特异性以及对监测低水平生物标志物的低毒性。
