Mastropasqua Rodolfo, Nubile Mario, Salgari Niccolò, Lanzini Manuela, Calienno Roberta, Mattei Peter A, Sborgia Alessandra, Agnifili Luca
Ophthalmology Unit, Department of Neurological, Neuropsychological, Morphological and Movement Sciences, University of Verona, Verona, Italy.
National Centre of High Technology (CNAT) in Ophthalmology of University "G. d'Annunzio", Chieti-Pescara, Italy.
PLoS One. 2017 Jun 1;12(6):e0178397. doi: 10.1371/journal.pone.0178397. eCollection 2017.
A rotating polarimetric 90°-cross linear-filter interferometry system was used to detect the morphological characteristics and features of interference patterns produced in in-vivo corneal stroma in healthy human corneas of 23 subjects. The characteristic corneal isogyres presenting with an evident cross-shaped pattern, grossly aligned with the fixation axis, were observed in all patients with centers within the pupillary dark area, impeding the exact determination of the center point. During the rotational scan in 78.3% of the eyes the cross-shaped pattern of the isogyre gradually separated to form two distinct hyperbolic arcs in opposite quadrants, reaching their maximal separation at 45 degrees with respect to angle of cross-shaped pattern formation. The corneal cross and hyperbolic-pattern repeated every 90° throughout the 360° rotational scan. While the interpretation of the isogyres presents particular difficulties, two summary parameters can be extracted for each cornea: the presence/orientation of a single or two dark areas in post-processed images and isochromes. However, the development of dedicated software for semi-quantitative analysis of these parameters and enantiomorphism may become available in the near future. The possible application of polarimetric interferometry in the field of both corneal pathologies and corneal surgery may be of great interest for clinical purposes.
使用旋转偏振90°交叉线性滤光干涉测量系统检测23名受试者健康人角膜体内角膜基质中产生的干涉图样的形态特征。在所有患者中均观察到特征性的角膜等视线呈现明显的十字形图案，大致与注视轴对齐，其中心位于瞳孔暗区内，妨碍了中心点的精确测定。在78.3%的眼睛旋转扫描过程中，等视线的十字形图案逐渐分开，在相对象限形成两个明显的双曲线弧，相对于十字形图案形成角度在45度时达到最大分离。在360°旋转扫描过程中，角膜十字和双曲线图案每90°重复一次。虽然对等视线的解释存在特殊困难，但可以为每个角膜提取两个汇总参数：后处理图像和等色线中单个或两个暗区的存在/方向。然而，用于这些参数和对映体半定量分析的专用软件可能在不久的将来问世。偏振干涉测量在角膜病理学和角膜手术领域的可能应用可能对临床目的具有极大的意义。
