Rustin G J, Peters T J
Br J Haematol. 1979 Apr;41(4):533-43. doi: 10.1111/j.1365-2141.1979.tb05891.x.
The organelle pathology of neutrophils in chronic granulocytic leukaemia (CGL) was investigated by analytical subcellular fractionation. There were minor reductions in activity of some granule enzymes with an abnormal distribution in sucrose density gradients of the specific granules. There was a marked reduction of 5'-nucleotidase activity but this is probably related to the relative reduction of the mononuclear cell contamination of the neutrophils isolated from leukaemic patients compared with controls. Another plasma membrane enzyme, NADH-nitroblue tetrazolium reductase, which has a microbicidal role, had increased activity. Neutrophils from patients with CGL had 13% the alkaline phosphatase activity of controls and were compared with neutrophils from women in the third trimester of pregnancy when the activity was increased to 8 times the control level. The latent activity, per cent inhibition by Levamisole, kinetic constants and subcellular distribution of alkaline phosphatase were similar in the three groups. It is suggested that the properties and intracellular localization of alkaline phosphatase are normal in CGL and that there is a quantitative lack of enzyme.
通过分析亚细胞分级分离法对慢性粒细胞白血病（CGL）中性粒细胞的细胞器病理学进行了研究。一些颗粒酶的活性略有降低，特异性颗粒在蔗糖密度梯度中的分布异常。5'-核苷酸酶活性显著降低，但这可能与白血病患者分离出的中性粒细胞与对照组相比单核细胞污染相对减少有关。另一种具有杀菌作用的质膜酶NADH-硝基蓝四唑还原酶活性增加。CGL患者的中性粒细胞碱性磷酸酶活性为对照组的13%，并与妊娠晚期女性的中性粒细胞进行了比较，此时该活性增加到对照组水平的8倍。三组中碱性磷酸酶的潜在活性、左旋咪唑的抑制百分比、动力学常数和亚细胞分布相似。提示CGL中碱性磷酸酶的性质和细胞内定位正常，只是存在酶的定量缺乏。
