一种测定人血浆和白细胞中吡哆醛及磷酸吡哆醛的荧光法及其在铁粒幼细胞性贫血患者中的应用
A fluorimetric method for the measurement of pyridoxal and pyridoxal phosphate in human plasma and leucocytes, and its application to patients with sideroblastic marrows.
作者信息
Smith G P, Samson D, Peters T J
出版信息
J Clin Pathol. 1983 Jun;36(6):701-6. doi: 10.1136/jcp.36.6.701.
Abstract
A highly sensitive fluorimetric assay for the measurement of pyridoxal and pyridoxal phosphate in biological tissues is described. The method involves the enzymic hydrolysis of pyridoxal phosphate to pyridoxal. The pyridoxal (free or total) is separated on an anion-exchange column, concentrated by cation-exchange chromatography and reacted with potassium cyanide under slightly alkaline conditions to form 4-pyridoxolactone, a highly fluorescent compound. The method is applied to the measurement of pyridoxal, pyridoxal phosphate and total pyridoxal in plasma and neutrophils from control subjects and patients with sideroblastic marrow and identified the patient with pyridoxine-responsive sideroblastic anaemia.
摘要
本文描述了一种用于测定生物组织中吡哆醛和磷酸吡哆醛的高灵敏度荧光测定法。该方法包括将磷酸吡哆醛酶解为吡哆醛。将吡哆醛（游离的或总的）在阴离子交换柱上分离，通过阳离子交换色谱法浓缩，并在微碱性条件下与氰化钾反应形成4-吡哆醇内酯，一种高荧光化合物。该方法应用于测定对照组、铁粒幼细胞性骨髓患者的血浆和中性粒细胞中的吡哆醛、磷酸吡哆醛和总吡哆醛，并鉴定出对吡哆醇反应性铁粒幼细胞性贫血患者。
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A fluorimetric method for the measurement of pyridoxal and pyridoxal phosphate in human plasma and leucocytes, and its application to patients with sideroblastic marrows.一种测定人血浆和白细胞中吡哆醛及磷酸吡哆醛的荧光法及其在铁粒幼细胞性贫血患者中的应用
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Arteriosclerotic lesions in pyridoxine-deficient monkeys.缺乏维生素B6的猴子的动脉硬化病变
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