干细胞与中风——发光的神经元如何照亮新路径。
Stem cells and stroke-how glowing neurons illuminate new paths.
作者信息
Mitrečić Dinko, Alić Ivan, Gorup Dunja
机构信息
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Zagreb, Croatia.
Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
出版信息
Neurogenesis (Austin). 2017 May 16;4(1):e1304847. doi: 10.1080/23262133.2017.1304847. eCollection 2017.
Abstract
A reliable method of cell tracing is essential in evaluating potential therapeutic procedures based on stem cell transplantation. Here we present data collected using neural stem cells isolated from a transgenic mouse line Thy1-YFP. When transplanted into a stroke affected brain these cells give rise to neurons that express a fluorescent signal which can be used for their detection and tracing. Observed processes were compared with those taking place during normal embryonic neurogenesis as well as during differentiation. Since the same neurogenic patterns were observed, we confirm that neural stem cell transplantation fits well into the paradigm of neuronal birth and differentiation.
摘要
在评估基于干细胞移植的潜在治疗方法时，可靠的细胞追踪方法至关重要。在此，我们展示了使用从转基因小鼠品系Thy1-YFP分离的神经干细胞收集的数据。当这些细胞被移植到中风后的大脑中时，它们会产生表达荧光信号的神经元，该信号可用于其检测和追踪。将观察到的过程与正常胚胎神经发生以及分化过程中发生的过程进行了比较。由于观察到相同的神经发生模式，我们证实神经干细胞移植非常符合神经元产生和分化的模式。
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