Allam Ahmed A, Gabr Sami A, Ajarem Jamaan, Alghadir Ahmad H, Sekar Revathi, Chow Billy Kc
Zoology Department, College of Science, King Saud University P.O.Box 2455, Riyadhll451, Saudia Arabia.
Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef 65211, Egypt.
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):166-176. doi: 10.21010/ajtcam.v14i2.18. eCollection 2017.
This study aims to examine the protective effect of green tea on the disturbances in oxidative stress and apoptosis related factors, mostly produced due to perinatal lipopolysaccharide (LPS) exposure, that subsequently induces liver cell damage.
Anti-free radical, Antioxidant, scavenging, geno-protective, and antiapoptotic activity of aqueous green tea extract (AGTE) were assessed against LPS-induced hepatic dysfunction in newborn-rats. AGTE at doses of 100 & 200 mg/kg was orally administered daily to rat dams, during gestation and lactation.
AGTE was observed to exhibit protective effects by significantly attenuating LPS-induced alterations in serum AST, ALT, bilirubin, and albumin levels. Significant increase in the total antioxidant capacity (TAC), DNA contents, and reduction in nitric oxide (NO) levels were observed in AGTE treated rats comparing LPS-toxicated ones. Additionally, AGTE treatment significantly down-regulated apoptotic markers and this effect was directly correlated to the degree of hepatic fibrosis. The possible mechanisms of the potential therapeutic-liver protective effect of AGTE could be due to free radical scavenging potential and antiapoptotic properties caused by the presence of antioxidant polyphenolic components in AGTE.
We thereby propose, based on our findings, that the anti-free radical and anti-apoptotic inducing properties of AGTE active constituents attribute to its functional efficacy as anti-fibrotic agent.
本研究旨在探讨绿茶对氧化应激和凋亡相关因子紊乱的保护作用，这些紊乱主要是由于围产期暴露于脂多糖（LPS）所致，随后会诱导肝细胞损伤。
评估了绿茶水提取物（AGTE）对新生大鼠LPS诱导的肝功能障碍的抗自由基、抗氧化、清除、基因保护和抗凋亡活性。在妊娠和哺乳期，每天给大鼠母鼠口服剂量为100和200mg/kg的AGTE。
观察到AGTE通过显著减轻LPS诱导的血清AST、ALT、胆红素和白蛋白水平的变化而发挥保护作用。与LPS中毒的大鼠相比，AGTE处理的大鼠总抗氧化能力（TAC）、DNA含量显著增加，一氧化氮（NO）水平降低。此外，AGTE处理显著下调凋亡标志物，且这种作用与肝纤维化程度直接相关。AGTE潜在的治疗性肝保护作用的可能机制可能是由于AGTE中抗氧化多酚成分的存在导致的自由基清除潜力和抗凋亡特性。
基于我们的研究结果，我们因此提出，AGTE活性成分的抗自由基和抗凋亡诱导特性归因于其作为抗纤维化剂的功能功效。
