Sukovatykh B S, Orlova A Iu
Chair of General Surgery, Kursk State Medical University, Kursk, Russia.
Angiol Sosud Khir. 2017;23(1):43-50.
The objective of the study was to experimentally substantiate a possibility of treating lower limb chronic ischaemia by means of administration of bone marrow cells. The study was performed on 100 laboratory 'Wistar' rats subdivided into four groups: intact, control, first and second study groups. The intact group consisted of 10 animals, with the remaining three containing 30 rats each. All animals, except the intact ones, were subjected to modelled critical ischaemia of a hind limb by means of excision of major vessels of the extremity. The rats from the first study group were given 'Myelopid' (calf bone marrow extract) at a dose of 50 μg/kg injected into the femoral muscles 3 hours after the operation, then after 1, 2 and 3 days. The second study group rats underwent cellular therapy with the mononuclear fraction of bone marrow autologous cells. Bone marrow was procured from the femoral bone of the contralateral limb. The exfusate was subjected to double centrifugation followed by isolation of the mononuclear fraction of the bone marrow according to the Boyum technique. The obtained mononuclear fraction of autologous cells of the bone marrow amounting to 4×106 cells in a volume of 200 μl was injected into the ischaemized limb of the animal. The control group received no treatment at all. In the first study group, the level of microcirculation as compared with the control group on day 10 increased by 48.3%, on day 21 by 50.6%, and on day 28 by 105.9%. In the second study group, the level of microcirculation as compared with the control group on day 10 increased by 67.4%, on day 21 by 85.7%, and on day 28 by 97% (the differences between the two study groups were statistically insignificant). Neither had the area of muscular necrosis statistically significant differences between the study groups, decreasing on day 10 averagely by 14.75%, on day 21 by 6.5% as compared with the control group animals, to become completely reduced on day 28. A conclusion was drawn that 'Myelopid' and the mononuclear fraction of autologous cells of bone marrow exerted a positive effect on the level of microcirculation, the scope of necrotic lesion of muscular tissue in the ischaemized extremity of laboratory animals and may potentially be used for treatment of patients suffering from obliterating diseases of lower-limb arteries.
本研究的目的是通过给予骨髓细胞，从实验上证实治疗下肢慢性缺血的可能性。该研究以100只“Wistar”实验大鼠为对象，分为四组：正常组、对照组、第一研究组和第二研究组。正常组有10只动物，其余三组每组各有30只大鼠。除正常组动物外，其余所有动物均通过切除下肢主要血管来模拟后肢严重缺血。第一研究组的大鼠在术后3小时，然后在第1、2和3天，以50μg/kg的剂量将“髓磷脂”（小牛骨髓提取物）注射到股部肌肉中。第二研究组的大鼠接受自体骨髓单个核细胞的细胞治疗。骨髓取自对侧肢体的股骨。将渗出液进行两次离心，然后根据博伊姆技术分离骨髓单个核细胞。将获得的200μl体积中含4×10⁶个细胞的自体骨髓单个核细胞注射到动物的缺血肢体中。对照组未接受任何治疗。在第一研究组中，与对照组相比，第10天时微循环水平提高了48.3%，第21天时提高了50.6%，第28天时提高了105.9%。在第二研究组中，与对照组相比，第10天时微循环水平提高了67.4%，第21天时提高了85.7%，第28天时提高了97%（两个研究组之间的差异无统计学意义）。各研究组之间肌肉坏死面积也无统计学显著差异，与对照组动物相比，第10天时平均减少了14.75%，第21天时减少了6.5%，在第28天时完全消失。得出的结论是，“髓磷脂”和自体骨髓单个核细胞对实验动物缺血肢体的微循环水平、肌肉组织坏死病变范围产生了积极影响，可能有潜力用于治疗患有下肢动脉闭塞性疾病的患者。
